Apolipoprotein H Human ELISA Kit (ab108814)
Overview
- Product nameApolipoprotein H Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts
- Assay typeSandwich
- Sensitivity= 0.6 ng/ml
- Range0.625 ng/ml - 40 ng/ml
- Recovery
99 %
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Apolipoprotein H (Apo H), previously known as ß2-glycoprotein I, is a 50 kDa plasma glycoprotein with 326 amino acids and circulates in plasma at about 200 µg/ml. ApoH inhibits the generation of factor Xa, XIa and XIIa, preventing activation of the intrinsic blood coagulation cascade. Binding of Apo H to anionic phospholipids such as phosphatidylserine and cardiolipin plays a key role in the formation of antiphospholipid antibodies, involving in autoimmune diseases like antiphospholipid syndrome or systemic lupus erythematosus. ApoH is increased in the plasma and liver of type 2 diabetic patients with metabolic syndrome and could be considered as a clinical marker of cardiovascular risk. ApoH interacts with viral proteins, such as the hepatitis B virus antigen, immunodeficiency virus type 1 and type 2 proteins, and Andes virus.
Ab108814 Human Apo H ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human Apo H in plasma, serum, urine, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Apo H in 4 hours. A polyclonal antibody specific for human Apo H has been pre-coated onto a 96-well microplate with removable strips. Apo H in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Apo H, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated Apo H Antibody 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Apo H Microplate 1 unit Human Apo H Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 30ml - Research Areas
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Alternative names
- Activated protein C binding proteinActivated protein C-binding proteinAnticardiolipin cofactor
- APC inhibitorApo-HAPOHAPOH_HUMANApolipoprotein HB2G1B2GPIBeta 2 glycoprotein IBeta 2 glycoprotein I precursor Beta(2)GPIBeta-2-glycoprotein 1Beta-2-glycoprotein IBGOTTMUSP00000003033
see all
- SwissProt: P02749 Human
Applications
Our Abpromise guarantee covers the use of ab108814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Apolipoprotein H Human ELISA Kit images
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ELISA - Apolipoprotein H Human ELISA Kit (ab108814)
Protocols
References for Apolipoprotein H Human ELISA Kit (ab108814)
ab108814 has not yet been referenced specifically in any publications.
