Anti-Apo-H antibody (ab203105)
Key features and details
- Rabbit polyclonal to Apo-H
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Apo-H antibody
See all Apo-H primary antibodies -
Description
Rabbit polyclonal to Apo-H -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Apo-H aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P02749 -
Positive control
- Human laryngeal cancer tissue; HepG2 cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
|
WB |
1/100 - 1/1000. Predicted molecular weight: 38 kDa.
|
Notes |
---|
IHC-P
1/100 - 1/500. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
WB
1/100 - 1/1000. Predicted molecular weight: 38 kDa. |
Target
-
Function
Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Contains 4 Sushi (CCP/SCR) domains. -
Post-translational
modificationsN- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 350 Human
- Entrez Gene: 11818 Mouse
- Entrez Gene: 287774 Rat
- Omim: 138700 Human
- SwissProt: P02749 Human
- SwissProt: Q01339 Mouse
- SwissProt: P26644 Rat
- Unigene: 445358 Human
see all -
Alternative names
- Activated protein C binding protein antibody
- Activated protein C-binding protein antibody
- Anticardiolipin cofactor antibody
see all
Images
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human laryngeal cancer tissue labeling Apo-H with ab203105 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human laryngeal cancer tissue labeling Apo-H with ab203105 at 1/200 dilution followed by Goat Anti-Rabbit IgG, AF488 conjugated, and DAPI staining.
-
Anti-Apo-H antibody (ab203105) at 1/300 dilution + HepG2 cell lysate
Predicted band size: 38 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab203105 has not yet been referenced specifically in any publications.