Anti-FIF antibody (ab99307)
Key features and details
- Rabbit polyclonal to FIF
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-FIF antibody
See all FIF primary antibodies -
Description
Rabbit polyclonal to FIF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
Immunogen
Synthetic peptide corresponding to Human FIF aa 1-50.
Database link: XP_508375.2 -
Positive control
- Whole cell lysates from HeLa, 293T and Mouse NIH 3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. -
Tissue specificity
Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer. -
Sequence similarities
Belongs to the API5 family. -
Domain
Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2. -
Cellular localization
Cytoplasm and Nucleus. Cytoplasm. Mainly nuclear. Can also be cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 8539 Human
- Entrez Gene: 11800 Mouse
- Omim: 609774 Human
- SwissProt: Q9BZZ5 Human
- SwissProt: O35841 Mouse
- Unigene: 435771 Human
- Unigene: 181824 Mouse
- Unigene: 402557 Mouse
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Alternative names
- AAC 11 antibody
- AAC-11 antibody
- AAC11 antibody
see all
Images
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All lanes : Anti-FIF antibody (ab99307) at 0.04 µg/ml
Lane 1 : HeLa whole cell
lysate at 50 µg
Lane 2 : HeLa whole cell
lysate at 15 µg
Lane 3 : HeLa whole cell
lysate at 5 µg
Lane 4 : 293T whole cell
lysate at 50 µg
Lane 5 : NIH 3T3 whole cell
lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling FIF with ab99307 at 1/1000 (0.2µg/mg) and 1/200 (1 µg/mg). Detection: DAB.
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ab99307, at 0.4 µg/ml, staining FIF in HeLa whole cell lysate immunoprecipitated using ab99307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 1 second.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab99307 has not yet been referenced specifically in any publications.