Apoptotic Cell Enrichment Kit (ab65782)
- Product nameApoptotic Cell Enrichment Kit
- Tests30 x 1 assay
- Sample typeAdherent cells, Suspension cells
- Assay typeDirect
- Assay time1h 00m
- Product overview
Abcam's Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca2+-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads adhere to the magnet, while non-apoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from healthy cell culture.
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- Tested applicationsFluorescence Microscopy, Functional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 30 tests 1x Binding Buffer 1 x 25ml Annexin V-Biotin Yellow 1 x 150µl Apoptotic Cell Releasing Buffer WM 1 x 10ml Streptavidin-MagBeads Brown 1 x 1.5ml
- Research Areas
Our Abpromise guarantee covers the use of ab65782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Fluorescence Microscopy||Fluorescence Microscopy|
References for Apoptotic Cell Enrichment Kit (ab65782)
ab65782 has not yet been referenced specifically in any publications.