Apoptotic Cell Enrichment Kit (ab65782)

Overview

• Product nameApoptotic Cell Enrichment Kit
• Tests
  30 x 1 assay
• Sample type
  Adherent cells, Suspension cells
• Assay typeDirect
• Assay time
  1h 00m
• Product overview

  Abcam's Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca2+-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads adhere to the magnet, while non-apoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from healthy cell culture.
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• Tested applicationsFluorescence Microscopy, Functional Studies more details

Properties

• Storage instructionsStore at -20°C. Please refer to protocols.

• Components	Identifier	30 tests
  1x Binding Buffer		1 x 25ml
  Annexin V-Biotin	Yellow	1 x 150µl
  Apoptotic Cell Releasing Buffer	WM	1 x 10ml
  Streptavidin-MagBeads	Brown	1 x 1.5ml

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • Apoptosis Kits
  • Apoptotic Cell Isolation Kits

  • Tags & Cell Markers
  • Cell Type Markers
  • Other Cell Types

  • Cell Biology
  • Other Antibodies
  • Other Antibodies

Applications

Protocols

Protocol Booklet