Overview

  • Product name
    Apoptotic DNA Ladder Detection Kit
    See all DNA fragments kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Direct
  • Assay time
    1h 30m
  • Product overview

    Apoptotic DNA Ladder Detection Kit (ab66090) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes, whereas other similar products in the market require 1-2 days. No DNA extraction or additional columns required.



    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab66090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Functional assays: Apoptotic DNA Ladder Detection Kit (ab66090)
    Functional assays: Apoptotic DNA Ladder Detection Kit (ab66090)

    Apoptosis was induced in 10e6 Jurkat cells by treatment with 10 μM Camptothecin (CPT) (ab120115) for 4 hours (2, 5) or 2 μM CPT for 24 hours (3, 6) whilst control cells were kept without treatment (1, 4). DNA was extracted and resuspended in 60 uL resuspension buffer. 10 uL (1-3) or 40 uL (4-6) were loaded onto the agarose gel.

Protocols

References

This product has been referenced in:
  • Inoue M  et al. Japanese apricot extract (MK615) potentiates bendamustine-induced apoptosis via impairment of the DNA damage response in lymphoma cells. Oncol Lett 14:792-800 (2017). Read more (PubMed: 28693235) »

See 1 Publication for this product

Publishing research using ab66090? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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7 reviews or Q&A

10
Votes: Highest First

Should the overnight step with enzyme B be incubated at 50 or 4 degrees Celsius overnight...and is it recommended for better results?

If you choose to do an overnight incubation with Enzyme B, we recommend doing so at 50 degrees Celsius. If you do this, make sure the tube is sealed well to prevent evaporation.



As long as you perform the incubation for at least 30 m...

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It is very easy to use.

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
very good for menuscript data
abreview image
Username

Miss. Jae Ok Seo

Verified customer

Submitted Jul 23 2014

Customer kindly contacted us to inquire about methods to resuspend the DNA pellet as they have been unable to do this by pipetting and vortexing. Further, they wished to inquire about the components of the DNA suspension buffer and if it contains a run...

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While it is uncommon for the DNA pellet to not break up with vortexing and pippetting, should this occur warming the solutions should help. There is also a yellow dye included in the suspension buffer for visualizing on the gel.

Customer kindly contacted us to inquire if the DNA pellet or resuspended DNA may be stored (frozen or 4C) and run later.

The lab has confirmed that you may store the resuspended DNA at -20°C. DNA generally does not freeze at this temp. Please don’t store it at 4°C.

Respected sir

I want the quotation for ab66090, Apoptotic DNA Ladder Detection Kit.
Thanking you

Thank you for your enquiry.

For India, you can order from one of our following official distributors. I can recommend to contact them and they can give you a price.

Central and South India:

Allied Scientific Products Read More

Phone to ask for tips and advice regarding this product. After addition and incubation of the buffers A and B the product is too viscous. This has made pelleting of the DNA impossible.

Thank you for contacting Abcam.

I have had the chance to speak with the lab. Although they have not seen this type of issue before, they suggest when doing overnight incubation to ensure that the tubes are airtight to prevent evaporation of ...

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Dear Technical support team Our customer wants to use ab66090. Before they purchase this item, they want to figure out the role of each reagent. Could you expain how the each component of the kit(Ammonium Acetate Solution/ DNA Suspension Buffer/ En...

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Thank you for your enquiry. I am pleased to confirm the following information for you regarding the components of this kit (ab66090) TE Lysis Buffer- Aids in cell lysis Enzyme A Solution- Removes RNA Enzyme B (Lyophilized)- Digests ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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