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Product name
Anti-Aquaporin 1 antibody
See all Aquaporin 1 products (10) ...
Description
Rabbit polyclonal to Aquaporin 1
Tested applications
ICC/IF, IHC-P, IF, WB, ELISA, IHC-Frmore details
Cross reactivity
Reacts with
Rat, Sheep, Dog, Human, Pig
Predicted to work with
Mouse, Cow
Immunogen
A 19 amino acid synthetic peptide within the carboxy terminal domain of rat Aquaporin 1 that is 100% homologous in mouse, rat, human and Bovine. This domain is predicted to be cytoplasmic.
Epitope
The epitope recognised by ab15080 is carboxy terminal and predicted to be cytoplasmic.
Properties
Form
Liquid
Storage instructions
Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 40% Glycerol, Whole Serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/500.
IF
IF: 1/500.IF: 1/500.
WB: 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.Can be blocked with Aquaporin 1 peptide (ab25381).
ELISA: 1/10000 - 1/50000.
IHC-Fr: 1/500.
Target
Function
Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Tissue specificity
Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Sequence similarities
Belongs to the MIP/aquaporin (TC 1.A.8) family.
Domain
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Cellular localization
Membrane.
Target information above from: UniProt accessionP29972
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AQP 1 antibody
- AQP CHIP antibody
- AQP-1 antibody
see all
Database links
- Entrez Gene: 282653 Cow
- Entrez Gene: 403732 Dog
- Entrez Gene: 358 Human
- Entrez Gene: 11826 Mouse
- Entrez Gene: 25240 Rat
- Entrez Gene: 442999 Sheep
- Omim: 107776 Human
- SwissProt: P47865 Cow
see all
Anti-Aquaporin 1 antibody images:
Immunohistochemistry (Frozen sections) - Aquaporin 1 antibody (ab15080)
ab15080 staining Aquaporin1 in porcine jejunum by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody diluted 1/500 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal IgG whole molecule to rabbit, diluted 1/400 was used as the secondary.
This image is courtesy of an Abreview submitted by Prof Marcin Arciszewski
Immunohistochemistry (Frozen sections) - Aquaporin 1 antibody (ab15080)
ab15080 at 1/500 dilution, staining Aquaporin 1 in sheep duodenum section by immunohistochemistry (Frozen sections). Sections were paraformaldehyde fixed and incubated with ab15080, for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit Ig, diluted 1/400 was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Prof Marcin Arciszewski
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aquaporin 1 antibody (ab15080)
ab15080 staining Aquaporin 1 in human kidney tubule tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde. Antigen retrieval was by heat mediation in citrate buffer (20 minutes at 100°C). Samples were incubated with primary antibody 1/2000(background reducer diluent) for 20 minutes at 25°C. An undiluted HRP polymer Goat anti mouse/rabbit monoclonal IgG was used as secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-Aquaporin 1 antibody (ab15080)
This product has been referenced in:
- Fossdal G et al. Aqp 9 and brain tumour stem cells. ScientificWorldJournal 2012:915176 (2012). ICC/IF.Read more (PubMed: 22262958) »
- Arciszewski MB et al. Aquaporin 1 water channel is expressed on submucosal but not myenteric neurons from the ovine duodenum. Ann Anat 193:81-5 (2011). IHC-Fr; Sheep.Read more (PubMed: 21168316) »
See all 6 publications for this product
Publishing research using ab15080? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"