Anti-Aquaporin 2 antibody - C-terminal (ab85876)

Overview

• Product nameAnti-Aquaporin 2 antibody - C-terminalSee all Aquaporin 2 primary antibodies ...
• Description
  Rabbit polyclonal to Aquaporin 2 - C-terminal
• Tested applicationsICC/IF, WB, IHC-P, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide designed within the C-terminal region of Human Aquaporin 2, conjugated to an immunogenic carrier protein

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Whole serum
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Metabolism
  • Plasma Membrane
  • Channels

Applications

Target

• FunctionForms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
• Tissue specificityExpressed in renal collecting tubules.
• Involvement in diseaseDefects in AQP2 are the cause of diabetes insipidus nephrogenic autosomal (ANDI) [MIM:125800]; also known as diabetes insipidus nephrogenic type 2. ANDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive.
• Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
• DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
• Post-translational
  modificationsSer-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
• Cellular localizationApical cell membrane. Cytoplasmic vesicle membrane. Shuttles from vesicles to the apical membrane.

Target information above from: UniProt accession P41181 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 359 Human
  • Entrez Gene: 11827 Mouse
  • Entrez Gene: 25386 Rat
  • Omim: 107777 Human
  • SwissProt: P41181 Human
  • SwissProt: P56402 Mouse
  • SwissProt: P34080 Rat
  • Unigene: 130730 Human

  • Unigene: 20206 Mouse
  • Unigene: 90076 Rat

  see all

• Alternative names
  ADH water channel antibodyAQP 2 antibodyAQP CD antibody

  AQP-2 antibodyAQP-CD antibodyAQP2 antibodyAQP2_HUMAN antibodyAQPCD antibodyAquaporin 2 collecting duct antibodyAquaporin CD antibodyAquaporin-2 antibodyAquaporin-CD antibodyAquaporin2 antibodyAquaporine 2 antibodyCollecting duct water channel protein antibodyMGC34501 antibodyWater channel aquaporin 2 antibodyWater channel protein for renal collecting duct antibodyWCH CD antibodyWCH-CD antibodyWCHCD antibody

  see all

Anti-Aquaporin 2 antibody - C-terminal images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aquaporin 2 antibody - C-terminal (ab85876)This image is courtesy of an Abreview submitted by Ross Benik
  ab85876 staining Aquaporin 2 - C-terminal in Rat kidney tissue sections by Immunohistochemistry (IHC-P - paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 25% serum free protein block for 5 minutes at 25°C; antigen retrieval was by heat mediation with a Tris/EDTA (pH 9.0) buffer. Samples were incubated with primary antibody (1/5000 in serum free antibody diluent) for 30 minutes. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Aquaporin 2 antibody - C-terminal (ab85876)
  ICC/IF image of ab85876 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85876, neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.