Overview

  • Product nameAnti-Aquaporin 2 antibody
    See all Aquaporin 2 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 2
  • Tested applicationsIHC-P, ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human, Pig
  • Immunogen

    A 15 aa synthetic MAP peptide (Rat) (C terminal). This peptide is predicted to be cytoplasmic.

Properties

Applications

Our Abpromise guarantee covers the use of ab15116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 21576461
ICC/IF 1/500.
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
ELISA 1/10000 - 1/50000.

Target

  • FunctionForms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificityExpressed in renal collecting tubules.
  • Involvement in diseaseDefects in AQP2 are the cause of diabetes insipidus nephrogenic autosomal (ANDI) [MIM:125800]; also known as diabetes insipidus nephrogenic type 2. ANDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive.
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    modifications
    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • Cellular localizationApical cell membrane. Cytoplasmic vesicle membrane. Shuttles from vesicles to the apical membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

References for Anti-Aquaporin 2 antibody (ab15116)

This product has been referenced in:
  • Dirocco DP  et al. Wnt4/ß-Catenin Signaling in Medullary Kidney Myofibroblasts. J Am Soc Nephrol 24:1399-412 (2013). Read more (PubMed: 23766539) »
  • Humphreys BD  et al. Repair of injured proximal tubule does not involve specialized progenitors. Proc Natl Acad Sci U S A 108:9226-31 (2011). IHC-P ; Mouse . Read more (PubMed: 21576461) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Sample Mouse Cell (mouse kidney TISSUE)
Specification mouse kidney TISSUE
Permeabilization Yes - triton-X
Fixative Acetone
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Submitted Aug 08 2013

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