Overview

  • Product name
    Aqueous Mounting Medium
    See all mounting media reagents
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-Pmore details
  • General notes
    Immobilizes coverglass over section, enhances FITC intensity and reduces fading to a significant degree.

    Reagent: 6 ml

    Procedure:

    FOR USE IN FLUORESCENCE MICROSCOPY

    After completion of immunostaining, remove slide from water, apply sufficient drops of Aqueous Mounting Medium upon section and mount coverglass. Coverslip should be immobilized within 30 minutes at room temperature. To avoid air bubbles within mounting medium, DO NOT SHAKE VIAL. If air bubbles are evident during coverslipping, carefully trim the vial tip with a sharp blade to create a larger opening and improve media flow. These directions are recommendations; however, they may be altered according to procedures established in-house.

    IHC Protocol Summary

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab128982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

References

This product has been referenced in:
  • Shaimerdenova M  et al. The effects of antiviral treatment on breast cancer cell line. Infect Agent Cancer 12:18 (2017). Read more (PubMed: 28344640) »
  • Dong C  et al. Ketamine Affects the Neurogenesis of Rat Fetal Neural Stem Progenitor Cells via the PI3K/Akt-p27 Signaling Pathway. Birth Defects Res B Dev Reprod Toxicol 101:355-63 (2014). Read more (PubMed: 25231110) »

See all 2 Publications for this product

Customer reviews and Q&As

nice item, fast shipping

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This mounting medium has very good quality. It does not cause bubbles if you are very careful and only use a small drop. My slides are still good several months after using the mounting medium.
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Abcam user community

Verified customer

Submitted Nov 23 2016

My pleasure as always. If I can be of any further help, please do not hesitate to ask.

Until then, have a lovely week.

Thank you for contacting us and your interest in our products.

For the experiment the customer is planning to perform, the BrightMount (ab103746) or BrightMount/Plus (ab103748) would be most appropriate and would be compatible with the fluores...

Read More

Thank you for contacting us.


The alternatives are as follows

1. ab103746; http://www.abcam.com/BrightMount-25ml-Aqueous-Mounting-Medium-for-Fluorescent-Staining-ab103746.html

or ab128982; http://www.abcam.com/Aqueous-Mou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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