Anti-Arg2 antibody (ab228700)
Key features and details
- Rabbit polyclonal to Arg2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Arg2 antibody
See all Arg2 primary antibodies -
Description
Rabbit polyclonal to Arg2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Rhesus monkey -
Immunogen
Recombinant fragment within Human Arg2 (internal sequence). The exact sequence is proprietary.
Database link: P78540 -
Positive control
- WB: NT2/D1 and PC-3 whole cell lysates; HEK-293T whole cell and cytoplasmic extracts. IHC-P: U373 xenograft tissue; mouse kidney and duodenum tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders. -
Tissue specificity
Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas. -
Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 518752 Cow
- Entrez Gene: 384 Human
- Entrez Gene: 11847 Mouse
- Omim: 107830 Human
- SwissProt: Q58DL1 Cow
- SwissProt: P78540 Human
- SwissProt: O08691 Mouse
- Unigene: 723133 Human
see all -
Alternative names
- ARG2 antibody
- ARGI2_HUMAN antibody
- Arginase II mitochondrial antibody
see all
Images
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All lanes : Anti-Arg2 antibody (ab228700) at 1/1000 dilution
Lane 1 : NT2/D2 (human carcinoma cell line from metastatic lung) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa10% SDS-PAGE
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Ice-cold methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Arg2 (green) using ab228700 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Paraffin-embedded mouse kidney tissue stained for Arg2 with ab228700 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-Arg2 antibody (ab228700) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T cytoplasmic extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa10% SDS-PAGE
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Paraffin-embedded mouse duodenum tissue stained for Arg2 with ab228700 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded U737 xenograft tissue stained for Arg2 with ab228700 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab228700 has been referenced in 2 publications.
- Banerjee B et al. Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism. Cell Mol Life Sci 79:41 (2021). PubMed: 34913090
- Atawia RT et al. Role of Arginase 2 in Murine Retinopathy Associated with Western Diet-Induced Obesity. J Clin Med 9:N/A (2020). PubMed: 31979105