Arg8-Vasopressin ELISA Kit (ab133028)
- Product nameArg8-Vasopressin ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Low conc 16 6.31pg/ml 10.2% Medium conc 16 56.4pg/ml 5.9% High conc 16 260.6pg/ml 10.6%
- Sample typeCell culture supernatant
- Assay typeCompetitive
- Sensitivity3.39 pg/ml
- Range4.1 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Tissue Culture Media 113.3 % - %
- Assay durationMultiple steps standard assay
- Species reactivity
Predicted to work with: a wide range of other species
- Product overview
The Arg8-Vasopressin ELISA kit is a competitive immunoassay for the quantitative determination of Vasopressin in samples. The kit uses a polyclonal antibody to Vasopressin to bind, in a competitive manner, the Vasopressin in the standards or sample or an alkaline phosphatase molecule which has Vasopressin covalently attached to it. After a simultaneous incubation at 4ºC the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Vasopressin in either standards or samples. The measured optical density is used to calculate the concentration of Vasopressin.
Arginine Vasopressin (AVP) is a 9 amino acid peptide with a 6-member disulfide ring. It is structurally related to oxytocin differing in 2 amino acids. It is synthesized in the hypothalamus supraoptic and paraventricular nuclei. It is stored in the posterior pituitary for release. AVP has powerful antidiuretic action and is also known as antidiuretic hormone (ADH). It acts upon the collecting tubule of the kidney increasing permeability to water and urea. It also has neurotransmitter and peripheral humoral functions.
AVP has been shown to be released upon both osmotic and non-osmotic stimuli, and its release into peripheral blood causes effects upon a number of factors, including emotional stress, posture, blood volume, and temperature. Alcohol appears to inhibit AVP secretion. Serum AVP measurement is used clinically for studies involving diabetes insipidus, syndrome of inappropriate ADH secretion (SIADH), ectopic AVP production and pyschogenic water intoxication.
Compound % Cross Reactivity Arg8-Vasopressin 100 Lys8-Vasopressin 3.6 Oxytocin <0.001 TRH <0.001 VIP <0.001 Leu-Enkephalin <0.001
<0.001 Mesotocine <0.001 Somatostatin <0.001 Vasotocin <0.001 Desmopressin <0.001
<0.001 Ser4,Ile8-Oxytocin <0.001
- Tested applicationsCompetitive ELISA more details
- Storage instructionsPlease refer to protocols.
Components 1 x 96 tests Assay Buffer 1 x 27ml Goat anti-Rabbit IgG Microtiter Plate 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml Vasopressin Antibody 1 x 5ml Vasopressin Conjugate 1 x 5ml Vasopressin Standard 1 x 0.5ml Wash Buffer Concentrate 1 x 30ml
- Research Areas
- Cellular localizationSecreted
- Antidiuretic hormone
- Arginine vasopressin
- Arginine vasopressin neurophysin II
- AVP NPII
- Vasopressin neurophysin 2 copeptin
- Vasopressin neurophysin II copeptin
Our Abpromise guarantee covers the use of ab133028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Arg8-Vasopressin ELISA Kit images
Typical results: This curve must not be used to calculate Vasopressin concentrations; each user must run a standard curve for each assay.
References for Arg8-Vasopressin ELISA Kit (ab133028)
ab133028 has not yet been referenced specifically in any publications.