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|Sample type||Average %||Range|
|Tissue Culture Media||113.3||% - %|
Abcam’s Arg8-Vasopressin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Arg8-Vasopressin in tissue culture media.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Arg8-Vasopressin antigen and a polyclonal rabbit antibody specific to Arg8-Vasopressin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Arg8-Vasopressin captured in the plate.
Arginine Vasopressin (AVP) is a 9 amino acid peptide with a 6-member disulfide ring. It is structurally related to oxytocin differing in 2 amino acids. It is synthesized in the hypothalamus supraoptic and paraventricular nuclei. It is stored in the posterior pituitary for release. AVP has powerful antidiuretic action and is also known as antidiuretic hormone (ADH). It acts upon the collecting tubule of the kidney increasing permeability to water and urea. It also has neurotransmitter and peripheral humoral functions.
AVP has been shown to be released upon both osmotic and non-osmotic stimuli, and its release into peripheral blood causes effects upon a number of factors, including emotional stress, posture, blood volume, and temperature. Alcohol appears to inhibit AVP secretion. Serum AVP measurement is used clinically for studies involving diabetes insipidus, syndrome of inappropriate ADH secretion (SIADH), ectopic AVP production and pyschogenic water intoxication.
|Compound||% Cross Reactivity|
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 27ml|
|Assay Buffer||1 x 27ml|
|Goat anti-rabbit IgG Microplate (12 x 8 wells)||1 unit|
|Plate Sealer||1 unit|
|pNpp Substrate||1 x 20ml|
|Stop Solution||1 x 5ml|
|Vasopressin Alkaline Phosphatase Conjugate||1 x 5ml|
|Vasopressin polyclonal rabbit Antibody||1 x 5ml|
|Vasopressin Standard||1 x 500µl|
Our Abpromise guarantee covers the use of ab133028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab133028 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"