Overview

  • Product nameArg8-Vasopressin ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Low conc 16 6.31pg/ml 10.2%
    Medium conc 16 56.4pg/ml 5.9%
    High conc 16 260.6pg/ml 10.6%
  • Sample type
    Cell culture supernatant
  • Assay typeCompetitive
  • Sensitivity
    3.39 pg/ml
  • Range
    4.1 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Culture Media 113.3 % - %

  • Assay durationMultiple steps standard assay
  • Species reactivity

    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s Arg8-Vasopressin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Arg8-Vasopressin in tissue culture media.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated- Arg8-Vasopressin antigen and a polyclonal rabbit antibody specific to Arg8-Vasopressin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Arg8-Vasopressin captured in the plate.

  • Notes

    Arginine Vasopressin (AVP) is a 9 amino acid peptide with a 6-member disulfide ring. It is structurally related to oxytocin differing in 2 amino acids. It is synthesized in the hypothalamus supraoptic and paraventricular nuclei. It is stored in the posterior pituitary for release. AVP has powerful antidiuretic action and is also known as antidiuretic hormone (ADH). It acts upon the collecting tubule of the kidney increasing permeability to water and urea. It also has neurotransmitter and peripheral humoral functions.

    AVP has been shown to be released upon both osmotic and non-osmotic stimuli, and its release into peripheral blood causes effects upon a number of factors, including emotional stress, posture, blood volume, and temperature. Alcohol appears to inhibit AVP secretion. Serum AVP measurement is used clinically for studies involving diabetes insipidus, syndrome of inappropriate ADH secretion (SIADH), ectopic AVP production and pyschogenic water intoxication.

    Cross Reactivity

    Compound% Cross Reactivity
    Arg8-Vasopressin100
    Lys8-Vasopressin3.6
    Oxytocin<0.001
    TRH<0.001
    VIP<0.001
    Leu-Enkephalin<0.001

    Met-Enkephalin

    <0.001
    Mesotocine<0.001
    Somatostatin<0.001
    Vasotocin<0.001
    Desmopressin<0.001

    Arg8-Vasotocin

    <0.001
    Ser4,Ile8-Oxytocin<0.001
  • Tested applicationsCompetitive ELISAmore details
  • PlatformMicroplate

Properties

  • Cellular localizationSecreted
  • Alternative names
    • ADH
    • Antidiuretic hormone
    • Arginine vasopressin
    • Arginine vasopressin neurophysin II
    • ARVP
    • AVP
    • AVP NPII
    • AVRP
    • Neurohypophyseal
    • Vasopressin neurophysin 2 copeptin
    • Vasopressin neurophysin II copeptin
    • VP
    see all

Applications

Our Abpromise guarantee covers the use of ab133028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Arg8-Vasopressin ELISA Kit images

  • Representative Standard Curve using ab133028

Protocols

References for Arg8-Vasopressin ELISA Kit (ab133028)

ab133028 has not yet been referenced specifically in any publications.

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We have not specifically validated this kit for serum or plasma but that doesn’t necessary mean that it would not work. As long as there is enough material for the kit to detect and ...

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The kit itself is not species specific because vasopressin is conserved between species. However, the plate in the kit is a goat anti-rabbit IgG so sample such as rabbit serum or plasma which will contain endogeno...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"