Arginase Activity Assay Kit (Colorimetric) (ab180877)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.2 U/ml
Overview
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Product name
Arginase Activity Assay Kit (Colorimetric)
See all Liver Arginase kits -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
< 0.2 U/ml -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Arginase Activity Assay Kit (Colorimetric) ab180877 is a simple, sensitive and rapid assay to quantify arginase activity.
In the arginase assay protocol, arginase reacts with arginine and undergoes a series of reactions that form an intermediate that react stoichiometrically with the probe to generate a colored product that can be detected at OD = 570 nm.
The kit can detect less than 0.2 U/L Arginase activity in 96-well assay format.
Arginase assay protocol summary:
- add samples and standards to wells
- add arginase substrate mix to sample wells only
- incubate for 20 min
- add arginase assay reaction mix to all wells
- analyze with microplate reader in kinetic mode for 10-30 min -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K755 Arginase Activity Colorimetric Assay Kit. K755-100 is the same size as the 100 test size of ab180877.
Arginase (EC 3.5.3.1) is the final enzyme of the Urea Cycle. It converts L-arginine into urea and L-ornithine and plays an important role in removing ammonium ion from the body.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Arginase Positive Control 1 vial Arginine 1 vial Assay Buffer XIV 1 x 25ml Converter Enzyme V 1 vial Developer I 1 vial Development Enzyme Mix I 1 vial H2O2 Standard 1 x 100µl OxiRed Probe 1 x 200ml -
Research areas
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Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- A I
- Al
- ARG 1
see all
Associated products
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Related Products
Images
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab180877 has been referenced in 19 publications.
- Zhang H et al. Annexin A2/TLR2/MYD88 pathway induces arginase 1 expression in tumor-associated neutrophils. J Clin Invest 132:N/A (2022). PubMed: 36377658
- Xu Q et al. Dynamic changes in myeloid-derived suppressor cells during the menstrual cycle: A pilot study. Front Med (Lausanne) 9:940554 (2022). PubMed: 36457573
- Ajina R et al. Antitumor T-cell Immunity Contributes to Pancreatic Cancer Immune Resistance. Cancer Immunol Res 9:386-400 (2021). PubMed: 33509790
- Vu LD et al. Deficiency in ST2 signaling ameliorates RSV-associated pulmonary hypertension. Am J Physiol Heart Circ Physiol 321:H309-H317 (2021). PubMed: 34170196
- Liu Y et al. Lactiplantibacillus plantarum Reduced Renal Calcium Oxalate Stones by Regulating Arginine Metabolism in Gut Microbiota. Front Microbiol 12:743097 (2021). PubMed: 34630369