Anti-Argininosuccinate Lyase antibody (ab67512)

Overview

• Product nameAnti-Argininosuccinate Lyase antibodySee all Argininosuccinate Lyase primary antibodies ...
• Description
  Mouse polyclonal to Argininosuccinate Lyase
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length argininosuccinate lyase protein (Human)

• Positive controlArgininosuccinate Lyase transfected 293T cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Signal Transduction
  • Metabolism
  • Amino Acids

Applications

Target

• PathwayAmino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
  Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1.
• Involvement in diseaseDefects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness.
• Sequence similaritiesBelongs to the lyase 1 family. Argininosuccinate lyase subfamily.
• Post-translational
  modificationsAcetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity.

Target information above from: UniProt accession P04424 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 435 Human
  • Omim: 608310 Human
  • SwissProt: P04424 Human
  • Unigene: 632015 Human

• Alternative names
  Argininosuccinase antibodyArgininosuccinate lyase antibodyArginosuccinase antibody

  ARLY_HUMAN antibodyASAL antibodyASL antibodyEC 4.3.2.1 antibody

  see all

Anti-Argininosuccinate Lyase antibody images

• 

  Western blot - Argininosuccinate Lyase antibody (ab67512)
  All lanes : Anti-Argininosuccinate Lyase antibody (ab67512) at 1/500 dilution
  
  Lane 1 : Argininosuccinate Lyase transfected 293T cell lysate
  Lane 2 : Non transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa