Anti-Aromatase antibody [EPR4534(2)] (ab124776)
- Product nameAnti-Aromatase antibody [EPR4534(2)]See all Aromatase primary antibodies ...
- DescriptionRabbit monoclonal [EPR4534(2)] to Aromatase
- Tested applicationsWB, IP, Flow Cyt more details
- Species reactivityReacts with: Rat, Human
Does not react withMouse
Synthetic peptide corresponding to residues in Aromatase (Human)
- Positive control
- WB: Human placenta, Human uterus, JAR or NIH:OVCAR 3 lysates Flow Cyt: Permeabilized NIH:OVCAR 3 cells
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Dissociation constant (KD) KD = 2.30 x 10 -12 M Learn more about KD
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR4534(2)
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab124776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).|
|IP||IP: 1/10 - 1/100.|
|Flow Cyt||Flow Cyt: 1/100 - 1/500.|
- FunctionCatalyzes the formation of aromatic C18 estrogens from C19 androgens.
- Tissue specificityBrain, placenta and gonads.
- Involvement in diseaseDefects in CYP19A1 are a cause of aromatase excess syndrome (AEXS) [MIM:139300]; also known as familial gynecomastia. AEXS is characterized by an estrogen excess due to an increased aromatase activity.
Defects in CYP19A1 are the cause of aromatase deficiency (AROD) [MIM:107910]. AROD is a rare disease in which fetal androgens are not converted into estrogens due to placental aromatase deficiency. Thus, pregnant women exhibit a hirsutism, which spontaneously resolves after post-partum. At birth, female babies present with pseudohermaphroditism due to virilization of extern genital organs. In adult females, manifestations include delay of puberty, breast hypoplasia and primary amenorrhoea with multicystic ovaries.
- Sequence similaritiesBelongs to the cytochrome P450 family.
- Cellular localizationMembrane.
- ARO antibody
- ARO1 antibody
- Aromatase antibody
- CP19A_HUMAN antibody
- CPV1 antibody
- CYAR antibody
- CYP19 antibody
- Cyp19a1 antibody
- CYPXIX antibody
- Cytochrome P-450AROM antibody
- Cytochrome P450 19A1 antibody
- Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody
- Cytochrome P450, subfamily XIX (aromatization of androgens) antibody
- Estrogen synthase antibody
- Estrogen synthetase antibody
- Flavoprotein linked monooxygenase antibody
- MGC104309 antibody
- Microsomal monooxygenase antibody
- OTTHUMP00000162543 antibody
- OTTHUMP00000198350 antibody
- P 450AROM antibody
Anti-Aromatase antibody [EPR4534(2)] images
All lanes : Anti-Aromatase antibody [EPR4534(2)] (ab124776) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human uterus lysate
Lane 3 : JAR lysate
Lane 4 : NIH:OVCAR 3 lysate
Lysates/proteins at 10 µg per lane.
HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size : 58 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
ab124776 (red), at 1/100, staining Arotamase in permeabilized NIH:OVCAR 3 cells. Rabbit IgG was used as a negative control (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
References for Anti-Aromatase antibody [EPR4534(2)] (ab124776)
ab124776 has not yet been referenced specifically in any publications.