![Immunocytochemistry/ Immunofluorescence - Arp2 antibody [FMS96] (ab49674) image](#arp2.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
Overview
Product name
Anti-Arp2 antibody [FMS96]
See all Arp2 products (6) ...
Description
Mouse monoclonal [FMS96] to Arp2
Specificity
This antibody recognizes Arp2 (approx. 40-45 kDa).
Tested applications
Indirect ELISA, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog, Human
Immunogen
Recombinant full length protein (Human)
Positive control
Total cell extract of C2 mouse myoblast cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
FMS96
Myeloma
NS1
Isotype
IgG1
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Crosslinking
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Immunocytochemistry/ Immunofluorescence - Arp2 antibody [FMS96] (ab49674)
![Immunocytochemistry/ Immunofluorescence - Arp2 antibody [FMS96] (ab49674) image](/ps/datasheet/Images/49/ab49674/arp2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab49674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
I-ELISA: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 10 µg/ml
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 40-45 kDa (predicted molecular weight: 45 kDa).
Target
Function
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.
Sequence similarities
Belongs to the actin family. ARP2 subfamily.
Cellular localization
Cytoplasm > cytoskeleton. Cell projection.
Target information above from: UniProt accessionP61160
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Actin like protein 2 antibody
- Actin related protein 2 antibody
- Actin-like protein 2 antibody
see all
Database links
- Entrez Gene: 10097 Human
- Entrez Gene: 66713 Mouse
- Entrez Gene: 289820 Rat
- Omim: 604221 Human
- SwissProt: P61160 Human
- SwissProt: P61161 Mouse
- SwissProt: Q5M7U6 Rat
- Unigene: 643727 Human
see all
Anti-Arp2 antibody [FMS96] images:
Immunocytochemistry/ Immunofluorescence - Arp2 antibody [FMS96] (ab49674)
C2C12 cells were incubated with ab49674 at 10 µg/ml followed by Goat Anti Mouse IgG Alexa-488 (green staining). The red staining is obtained by staining actin with Alexa-546 phalloidin.
References for Anti-Arp2 antibody [FMS96] (ab49674)
This product has been referenced in:
- Sun SCet al. Arp2/3 complex regulates asymmetric division and cytokinesis in mouse oocytes. PLoS One 6:e18392 (2011). ICC/IF; Mouse.Read more (PubMed: 21494665) »
- Codarin Eet al. Differential proteomic analysis of subfractioned human hepatocellular carcinoma tissues. J Proteome Res 8:2273-84 (2009).Read more (PubMed: 19290626) »
See all 2 publications for this product
Publishing research using ab49674? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"