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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab12099? Please let us know so that we can cite the reference in this datasheet
ab12099 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Arp 5 antibody (ab12099)
Anti-Arp5 antibody (ab12099) + S. cereviseae lysate
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size : 87 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa (possible cross reactivity).
Western blot - Arp5 antibody (ab12099)
All lanes : Anti-Arp5 antibody (ab12099) at 0.36 µg/ml
Lane 1 : S. cerevisiae whole cell lysate (wild type)
Lane 2 : S. cerevisiae whole cell lysate (arp5 knockout)
Lane 3 : S. cerevisiae whole cell lysate (arp8 knockout)
Lane 4 : S. cerevisiae whole cell lysate (ino80 knockout)
Lane 5 : S. cerevisiae whole cell lysate (anc1 knockout)
Lane 6 : S. cerevisiae whole cell lysate (nhp10 knockout)
Lane 7 : S. cerevisiae whole cell lysate (wild type, treated with MMS)
Lane 8 : S. cerevisiae whole cell lysate (arp8 knockout, treated with MMS)
Predicted band size : 87 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Using ab12099, bands were present in all S. cerevisiae strains tested except for an arp5 knockout strain. This provides conclusive evidence that the ab12099 recognises Arp5.
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