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Products:Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> INO80
Overview
Product name
Anti-Arp5 antibody
Description
Rabbit polyclonal to Arp5
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Recombinant full length protein Arp 5.
Positive control
S. cereviseae lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> INO80
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 5 µg/mlDetects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
Target
Relevance
The budding yeast INO80 complex is a conserved ATP-dependent nucleosome remodeler containing actin-related proteins Arp5 and Arp8. Strains lacking INO80, ARP5, or ARP8 have defects in transcription. Ino80, Arp5, and Arp8 are recruited to an HO-induced DSB, where a phosphorylated form of H2A accumulates. Recruitment of Ino80 is compromised in cells lacking the H2A phosphoacceptor S129. INO80-mediated chromatin remodeling seems to occur directly at DSBs, where it appears to facilitate processing of the lesion.
Cellular localization
Nuclear
Alternative names
- Actin-like protein ARP5 antibody
- ARP5_YEAST antibody
- P53946 antibody
Database links
- Entrez Gene: 855665 Saccharomyces cerevisiae
- SwissProt: P53946 Saccharomyces cerevisiae
Anti-Arp5 antibody images:
Western blot - Arp 5 antibody (ab12099)
Anti-Arp5 antibody (ab12099) + S. cereviseae lysate
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size : 87 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa (possible cross reactivity).
Western blot - Arp5 antibody (ab12099)
All lanes : Anti-Arp5 antibody (ab12099) at 0.36 µg/ml
Lane 1 : S. cerevisiae whole cell lysate (wild type)
Lane 2 : S. cerevisiae whole cell lysate (arp5 knockout)
Lane 3 : S. cerevisiae whole cell lysate (arp8 knockout)
Lane 4 : S. cerevisiae whole cell lysate (ino80 knockout)
Lane 5 : S. cerevisiae whole cell lysate (anc1 knockout)
Lane 6 : S. cerevisiae whole cell lysate (nhp10 knockout)
Lane 7 : S. cerevisiae whole cell lysate (wild type, treated with MMS)
Lane 8 : S. cerevisiae whole cell lysate (arp8 knockout, treated with MMS)
Predicted band size : 87 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Using ab12099, bands were present in all S. cerevisiae strains tested except for an arp5 knockout strain. This provides conclusive evidence that the ab12099 recognises Arp5.
References for Anti-Arp5 antibody (ab12099)
ab12099 has not yet been referenced specifically in any publications.
Publishing research using ab12099? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"