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Western blot - Arp8 antibody (ab12098)
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Product Name
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Arp8 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Arp8
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Immunogen
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Recombinant full length protein of Saccharomyces cerevisiae Arp 8.
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Reacts with
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Sc
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: Use at a concentration of 5 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Microbiology >> Organism >> Eukaryotic Microorganism >> Yeast >> Saccharomyces spp
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> INO80
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Relevance
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The budding yeast INO80 complex is a conserved ATP-dependent nucleosome remodeler containing actin-related proteins Arp5 and Arp8. Strains lacking INO80, ARP5, or ARP8 have defects in transcription. Arp8 is an actin related protein that is probably involved in transcription regulation via its interaction with the INO80 complex, a chromatin remodeling complex.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 854309 (Saccharomyces cerevisiae)
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| SwissProt | | Q12386 (Saccharomyces cerevisiae)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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ab12098 recognises a band of approximately 100kDa corresponding to Arp8 using purified INO80 complex. The antibody does not recognize Arp8 using S. pombe or S cerevisiae whole cell extracts but it is possible that this could be improved using different blocking conditions.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Arp8 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Arp8 antibody images, references, products related to ab12098 and other tools.
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Arp8 antibody images:
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 Western blot - Arp8 antibody (ab12098)
All lanes : Arp8 antibody (ab12098)
Lane 1 : FLAG-purification using S. pombe whole cell extract not expressing FLAG-tagged-INO80 complex
Lane 2 : FLAG-purification using S. pombe whole cell extract expressing FLAG-tagged-INO80 complex
Predicted band size : 100 kDa
Observed band size : 100 kDa
Additional bands at : 95 kDa (possible degradation product).
S. pombe FLAG-tagged-INO80 complex was immunoprecipitated (lane2) and immunoblotted using anti-Arp8 antibodies (ab12098).
Arp8 a member of the INO80 complex was detected as at approximately 100 kDa, which is predicted to be the higher band of the doublet. The lower band of the doublet may represent a proteolytic cleavage product.
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Search PubMed (MEDLINE) for references to Arp8
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Arp8 antibody - more information
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Customer reviews (feedback) regarding Arp8 antibody |
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Arp8 antibody - related products:
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