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Products:Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> INO80
Overview
Product name
Anti-Arp8 antibody
Description
Rabbit polyclonal to Arp8
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Recombinant full length protein of Saccharomyces cerevisiae Arp 8.
General notes
ab12098 recognises a band of approximately 100kDa corresponding to Arp8 using purified INO80 complex. The antibody does not recognize Arp8 using S. pombe or S cerevisiae whole cell extracts but it is possible that this could be improved using different blocking conditions.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Organism >> Eukaryotic Microorganism >> Yeast >> Saccharomyces spp
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> INO80
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Western blot - Arp8 antibody (ab12098)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 5 µg/mlDetects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
Target
Relevance
The budding yeast INO80 complex is a conserved ATP-dependent nucleosome remodeler containing actin-related proteins Arp5 and Arp8. Strains lacking INO80, ARP5, or ARP8 have defects in transcription. Arp8 is an actin related protein that is probably involved in transcription regulation via its interaction with the INO80 complex, a chromatin remodeling complex.
Cellular localization
Nuclear
Alternative names
- Actin like protein ARP8 antibody
- Actin-like protein ARP8 antibody
- ARP8_YEAST antibody
see all
Database links
- Entrez Gene: 854309 Saccharomyces cerevisiae
- SwissProt: Q12386 Saccharomyces cerevisiae
Anti-Arp8 antibody images:
Western blot - Arp8 antibody (ab12098)
All lanes : Anti-Arp8 antibody (ab12098)
Lane 1 : FLAG-purification using S. pombe whole cell extract not expressing FLAG-tagged-INO80 complex
Lane 2 : FLAG-purification using S. pombe whole cell extract expressing FLAG-tagged-INO80 complex
Predicted band size : 100 kDa
Observed band size : 100 kDa
Additional bands at : 95 kDa (possible degradation product).
S. pombe FLAG-tagged-INO80 complex was immunoprecipitated (lane2) and immunoblotted using anti-Arp8 antibodies (ab12098).
Arp8 a member of the INO80 complex was detected as at approximately 100 kDa, which is predicted to be the higher band of the doublet. The lower band of the doublet may represent a proteolytic cleavage product.
References for Anti-Arp8 antibody (ab12098)
ab12098 has not yet been referenced specifically in any publications.
Publishing research using ab12098? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"