Aryl hydrocarbon Receptor antibody [RPT1] (ab2770)

Product Name 

Aryl hydrocarbon Receptor antibody [RPT1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [RPT1] to Aryl hydrocarbon Receptor

Immunogen 

Synthetic Peptide: (12)RKRRKP (17)V(22)K PIPAEGIK(3
Immunizing peptide corresponds to amino acid residues 12-31 from the mouse AhR protein with amino acids 18-21 being omitted.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Detects the aryl hydrocarbon receptor (AhR).

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at an assay dependent dilution (PMID 19617203).
IP: Use at an assay dependent dilution. Although ab2770 can be used in immunoprecipitation procedures, clone RPT9 is recommended for this procedure.
WB: 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Helix-Turn-Helix

Relevance 

The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt). The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

RPT1

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Ascitic fluid with 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Aryl hydrocarbon Receptor antibody [RPT1] (ab2770)

  This product has been used in: (two most recent references)

Khuda-Bukhsh AR et al. Modulation of Signal Proteins: A Plausible Mechanism to Explain How a Potentized Drug Secale Cor 30C Diluted beyond Avogadro's Limit Combats Skin Papilloma in Mice. Evid Based Complement Alternat Med : (2009). IHC-P; Mouse. PubMed: 19617203

Gramatzki D et al. Aryl hydrocarbon receptor inhibition downregulates the TGF-beta/Smad pathway in human glioblastoma cells. Oncogene 28:2593-605 (2009). WB. PubMed: 19465936

See all 5 publication references for this product.


If you publish research using ab2770 please let us know so that we can cite the reference on this datasheet.

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