Anti-Aryl hydrocarbon Receptor antibody [RPT1] (ab2770)

Immunocytochemistry/ Immunofluorescence - Aryl hydrocarbon Receptor antibody [RPT1] (ab2770)

ICC/IF image of ab2770 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2770, at a 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Aryl hydrocarbon Receptor antibody [RPT1] (ab2770)

Predicted band size : 94 kDa


Lane 1 (from the left): protein marker.
Lane 2: IP sample with a rabbit anti-HSP90 antibody, un-treatment.
Lane 3: IP sample with a rabbit anti-HSP90 antibody treated with 10ng/ml Depsipeptide for 24 hours.
Lane 4: IP sample with a rabbit anti-HSP90 antibody treated with 10µm Benzo(a)pyrene for 24 hours.
Lane 5 - 7: No antibody IP samples the same order as lane 2-4.
Lane 8-10: Whole cell lysate without IP the same order as lane 2-4.

Primary antibody ab2770 used at a 1/1000 dilution, Goat anti-mouse (IRDye® 800CW) secondary antibody used at a 1/10000 dilution.

Image courtesy of an anonymous Abreview.

Flow Cytometry-Anti-Aryl hydrocarbon Receptor antibody [RPT1](ab2770)

Overlay histogram showing HEK293 cells stained with ab2770 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2770, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.