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Immunocytochemistry/ Immunofluorescence - Aryl hydrocarbon Receptor antibody [RPT9] (ab2769)
![Immunocytochemistry/ Immunofluorescence - Aryl hydrocarbon Receptor antibody [RPT9] (ab2769)](\ps\datasheet\images\2\ab2769\Aryl-hydrocarbon-Receptor-Primary-antibodies-ab2769-2.jpg)
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Product Name
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Aryl hydrocarbon Receptor antibody [RPT9]
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See all Aryl hydrocarbon Receptor antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [RPT9] to Aryl hydrocarbon Receptor
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Immunogen
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Synthetic Peptide: (12)RKRRKP (17)V(22)K PIPAEGIK(3 immunizing peptide corresponds to amino acid residues 12-31 from the mouse AhR protein with amino acids 18-21 being omitted.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody specifically immunoprecipitates a single ~95 kDa protein representing AHR from Hepa 1 cytosol. Immunohistochemical staining of AHR in rat liver results in strong cytoplasmic and some nuclear staining.
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Tested applications
(see key)
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ICC/IF, IHC-P, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: 1/100.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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rat liver tissue sections
Hepa 1 cytosolic lysate
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Cellular localization
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First cytoplasmic, upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Helix-Turn-Helix
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Relevance
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The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).
Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor. Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt). The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
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Aryl hydrocarbon Receptor antibody [RPT9] (ab2769) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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RPT9
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Isotype
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IgG
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Purity
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Ascites
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Storage buffer
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Ascitic fluid with 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Aryl hydrocarbon Receptor antibody [RPT9] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Aryl hydrocarbon Receptor antibody [RPT9] images, references, products related to ab2769 and other tools.
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Aryl hydrocarbon Receptor antibody [RPT9] images:
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 Immunocytochemistry/ Immunofluorescence - Aryl hydrocarbon Receptor antibody [RPT9] (ab2769)
ICC/IF image of ab2769 stained HepG2 cells. The cells were 5% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2769, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for Aryl hydrocarbon Receptor antibody [RPT9] (ab2769)
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This product has been used in: (two most recent references)
Gramatzki D et al. Aryl hydrocarbon receptor inhibition downregulates the TGF-beta/Smad pathway in human glioblastoma cells. Oncogene 28:2593-605 (2009). ICC/IF. PubMed: 19465936
Randi AS et al. Hexachlorobenzene triggers AhR translocation to the nucleus, c-Src activation and EGFR transactivation in rat liver. Toxicol Lett 177:116-22 (2008). PubMed: 18295415
See all 3 publication references for this product.
If you publish research using ab2769 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Aryl hydrocarbon Receptor
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Aryl hydrocarbon Receptor antibody [RPT9] - more information
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Aryl hydrocarbon Receptor antibody [RPT9] - related products:
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Aryl hydrocarbon Receptor antibody [RPT9] - other tools:
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Contact Abcam with a Technical Enquiry about Aryl hydrocarbon Receptor antibody [RPT9] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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