Anti-Aryl hydrocarbon Receptor antibody (ab49351)

Overview

  • Product nameAnti-Aryl hydrocarbon Receptor antibodySee all Aryl hydrocarbon Receptor primary antibodies ...
  • Description
    Rabbit polyclonal to Aryl hydrocarbon Receptor
  • SpecificityThis antibody recognizes Aryl hydrocarbon Receptor
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Cow, Dog
  • Immunogen

    Synthetic peptide: SVSYLRAKSF FDVALKSSPT ERNGGQDNCR AANFREGLNL QEGEFLLQAL , corresponding to amino acids 73-122 of Human Aryl hydrocarbon Receptor

  • Positive control
    • HepG2 cell lysate and human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab49351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 32, 96 kDa (predicted molecular weight: 96 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionLigand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
  • Tissue specificityExpressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localizationCytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ah receptor antibody
    • AhR antibody
    • AHR_HUMAN antibody
    • Aromatic hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor precursor antibody
    • bHLHe76 antibody
    • Class E basic helix loop helix protein 76 antibody
    • Class E basic helix-loop-helix protein 76 antibody
    • HGNC:348 antibody
    see all

Anti-Aryl hydrocarbon Receptor antibody images

  • Anti-Aryl hydrocarbon Receptor antibody (ab49351) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 96 kDa
    Observed band size : 32,96 kDa (why is the actual band size different from the predicted?)
  • ab49351 at 4µg/ml staining Aryl hydrocarbon Receptor in epithelial cells of renal tubule in human kidney tissue.

References for Anti-Aryl hydrocarbon Receptor antibody (ab49351)

ab49351 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic stem cell)
Loading amount 200000 cells
Specification embryonic stem cell
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Dec 17 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (embryonic stem cell)
Specification embryonic stem cell
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme-free
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy cells
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Submitted Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"