Anti-Aryl hydrocarbon Receptor antibody (ab49351)

Overview

  • Product nameAnti-Aryl hydrocarbon Receptor antibody
    See all Aryl hydrocarbon Receptor primary antibodies
  • Description
    Rabbit polyclonal to Aryl hydrocarbon Receptor
  • SpecificityThis antibody recognizes Aryl hydrocarbon Receptor
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Cow, Dog
  • Immunogen

    Synthetic peptide: SVSYLRAKSF FDVALKSSPT ERNGGQDNCR AANFREGLNL QEGEFLLQAL , corresponding to amino acids 73-122 of Human Aryl hydrocarbon Receptor

  • Positive control
    • HepG2 cell lysate and human kidney tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 32, 96 kDa (predicted molecular weight: 96 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionLigand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
  • Tissue specificityExpressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Cellular localizationCytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ah receptor antibody
    • AhR antibody
    • AHR_HUMAN antibody
    • Aromatic hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor antibody
    • Aryl hydrocarbon receptor precursor antibody
    • bHLHe76 antibody
    • Class E basic helix loop helix protein 76 antibody
    • Class E basic helix-loop-helix protein 76 antibody
    • HGNC:348 antibody
    see all

Anti-Aryl hydrocarbon Receptor antibody images

  • Anti-Aryl hydrocarbon Receptor antibody (ab49351) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 96 kDa
    Observed band size : 32,96 kDa (why is the actual band size different from the predicted?)
  • ab49351 at 4µg/ml staining Aryl hydrocarbon Receptor in epithelial cells of renal tubule in human kidney tissue.

References for Anti-Aryl hydrocarbon Receptor antibody (ab49351)

ab49351 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic stem cell)
Loading amount 200000 cells
Specification embryonic stem cell
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Dec 17 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (embryonic stem cell)
Specification embryonic stem cell
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme-free
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy cells
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Submitted Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"