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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Helix-Turn-Helix
Overview
Product name
Anti-Aryl hydrocarbon Receptor antibody
See all Aryl hydrocarbon Receptor products (7) ...
Description
Rabbit polyclonal to Aryl hydrocarbon Receptor
Specificity
This antibody recognizes Aryl hydrocarbon Receptor
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Sheep, Cow, Dog
Immunogen
Synthetic peptide: SVSYLRAKSF FDVALKSSPT ERNGGQDNCR AANFREGLNL QEGEFLLQAL , corresponding to amino acids 73-122 of Human Aryl hydrocarbon Receptor
SVSYLRAKSF FDVALKSSPT ERNGGQDNCR AANFREGLNL QEGEFLLQAL
Positive control
HepG2 cell lysate and human kidney tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Helix-Turn-Helix
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
IHC-P
Application notes
ELISA: Use at an assay dependent dilution. Titre using a peptide based assay: 312500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 2.5 µg/ml. Detects a band of approximately 32 & 96 kDa (predicted molecular weight: 96 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues.
Tissue specificity
Expressed in all tissues tested including blood, brain, heart, kidney, liver, lung, pancreas and skeletal muscle.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains.
Cellular localization
Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
Target information above from: UniProt accessionP35869
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ah receptor antibody
- AhR antibody
- AHR_HUMAN antibody
see all
Database links
- Entrez Gene: 280714 Cow
- Entrez Gene: 196 Human
- Omim: 600253 Human
- SwissProt: P35869 Human
- Unigene: 171189 Human
Anti-Aryl hydrocarbon Receptor antibody images:
Western blot - Aryl hydrocarbon Receptor antibody (ab49351)
Anti-Aryl hydrocarbon Receptor antibody (ab49351) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS.
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 96 kDa
Observed band size : 32,96 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Immunohistochemistry (Paraffin-embedded sections) - Aryl hydrocarbon Receptor antibody (ab49351)
ab49351 at 4µg/ml staining Aryl hydrocarbon Receptor in epithelial cells of renal tubule in human kidney tissue.
References for Anti-Aryl hydrocarbon Receptor antibody (ab49351)
ab49351 has not yet been referenced specifically in any publications.
Publishing research using ab49351? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"