Anti-Ascaris lumbricoides IgG Human ELISA Kit (ab108707)
- Product nameAnti-Ascaris lumbricoides IgG Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Pos. Serum 16 3.1% Neg. Serum 16 3.8%
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeIndirect
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s anti-Ascaris lumbricoides IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Ascaris lumbricoides in Human serum and plasma.
A 96-well plate has been precoated with Ascaris lumbricoides antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Ascaris lumbricoides-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ascaris lumbricoides IgG sample captured in plate.
- Tested applicationsIndirect ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Ascaris lumbricoides (IgG) Coated Microplate (12 x 8 wells) 1 unit Ascaris lumbricoides IgG Cut-off Control Green cap 1 x 3ml Ascaris lumbricoides IgG Negative Control Blue cap 1 x 2ml Ascaris lumbricoides IgG Positive Control Red cap 1 x 2ml Ascaris lumbricoides Protein A HRP Conjugate Black cap 1 x 20ml Cover foil 1 unit IgG Sample Diluent White cap 1 x 100ml Stop Solution Red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
- Research Areas
Our Abpromise guarantee covers the use of ab108707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Anti-Ascaris lumbricoides IgG Human ELISA Kit (ab108707)
ab108707 has not yet been referenced specifically in any publications.