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Ascorbic Acid Assay Kit (Biological Samples) (ab65656)

Q&A (16)Specific References (1)

Overview

  • Product nameAscorbic Acid Assay Kit (Biological Samples)See all Ascorbic acid kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • Assay typeQuantitative
  • Sensitivity
    > 0.2 nmol/well
  • Range
    0.2 nmol/well - 20 nmol/well
  • Assay time
    0h 30m
  • Product overview

    Abcam's Ascorbic Acid Assay Kit (Biological Samples) provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Fe3+ is reduced to Fe2+ by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.
    Visit our FAQs page for tips and troubleshooting.

     

    Suitable for biological samples
    Range: 0.2 to 20 nmol of ascorbic acid

  • Notes

    Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceAscorbic Acid (C6H8O6) is an essential nutrient in human diets, and is necessary to maintain connective tissue and bone. The biologically active form of ascorbic acid is vitamic C, a water soluble vitamin which is the L-enantiomer form of ascorbic acid. Humans have lost the ability to synthesize ascorbic acid and must obtain in the diet. Ascorbic acid is an electron donor for enzymes involved in collagen hydroxylation, biosynthesis of carnitine and norepinephrine, tyrosine metabolism and amidatin of peptide hormones. The ability of vitamin C to donate electrons also makes it a potent water-soluble anti-oxidant that readily scavenges free radicals.

    • Applications

    Our Abpromise guarantee covers the use of ab65656 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Ascorbic Acid Assay Kit (Biological Samples) images

    • Functional Studies - Ferric Reducing Ascorbate Assay Kit (ab65656)
      Functional Studies - Ferric Reducing Ascorbate Assay Kit (ab65656)
      Ascorbic Acid Standard Curve.

    Protocols

    References for Ascorbic Acid Assay Kit (Biological Samples) (ab65656)

    This product has been referenced in:
    • Dai D  et al. Healing of saccular aneurysms following platinum coil embolization: lack of improved efficacy with vitamin C supplementation. J Neurointerv Surg N/A:N/A (2012). Functional Studies ; Rabbit . Read more (PubMed: 22914744) »

    See 1 Publication for this product

    Publishing research using ab65656 ? Please let us know so that we can cite the reference in this datasheet

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    Displaying 1 - 10 of 16 results for Abreviews and Q&A

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    Would you please help me to find assay for Ascorbate Peroxidase Assay , is it same as ab65656?


    Unfortunately, this kit will measure the level of Vitamin C, Ascorbic Acid, in biological samples, but not the protein you are after. Ascorbate peroxidases (or APX1) are enzymes that detoxify peroxides such as hydrogen peroxide using ascorbate as a s...

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    Dear xxxxx,
    Please see attached excel file with example of data.
    In the first tab there is a raw data from the plate reader for 3 point calibration ( conc. 0, 5 and 10 nmols per well).
    It the second tab there is some samples data.
    I used ...

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    I am sorry for the delay in getting back to you. I now have further input from the lab.
    From the results you have shared it does seem like there is higher background in your samples than would be expected. This can often be improved by optimizing the ...

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    Phone call reporting the variation in the readings collected with the samples using the Ascorbic Acid Assay Kit (ab65656).

    Thank you for contacting us yesterday in regards to the Ascorbic Acid Assay Kit (ab65656). I was sorry to hear you have been having some difficulties in achieving consistent results with your samples.
    As discussed over the phone, I would like to take...

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    will use Hela Cells
    can 0.1% SDS be used for lysis and be compatible with kit?
    send composition of NP40 buffer

    Thank you for contacting us.

    The lab confirmed thata 0.1% SDS based lysis buffer can be used for cell lysis, and would be compatible with the kit.

    I hope this information is helpful to you. Please do not hesitate to contact us if you...

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    will use Hela Cells
    can 0.1% SDS be used for lysis and be compatible with kit?
    send composition of NP40 buffer

    Thank you for contacting us.

    Here is the buffer composition:

    Nonidet-P40 (NP-40) buffer:
    20 mM Tris HCl pH 8
    137 mM NaCl
    10% glycerol
    1% nonidet P-40
    2 mM EDTA

    I hope this information is helpful to y...

    Read More

    I saw in your Webpage that you provide the Ascorbic Acid Assay Kit for biological samples. (ab65656).
    We're thinking about using something like that in our experiments and I would like to ask if it could be possible to get afree-test kitfrom it for t...

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    Thank you for contacting us. Because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products and kits.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, whic...

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    May I please have a quote for 1 Ferric Reducing Ascorbate Assay Kit (ab 65656) and 1 package of 10 kD spin columns (ab 93349)? Also, we are planning on using this kit for an upcoming study. How many kits do you recommend purchasing if we wish t...

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    Thank you for your email. Each kit contains sufficient reagents for 100 tests, therefore it is possible to test ~40 samples in duplicate with each kit (6 data points in the standard curve). Therefore you will require 3 kits for 120 samples. ...

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    samples are frozen plasma with EDTA (not serum) about 3 samples of blood per patient (normal, before treatment, after treatment) 1. Can plasma samples be used (would plasma proteins interfere)? 2. If proteins interfere, can they be removed after thaw...

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    Thank you for your patience. Yes, I can confirm that in the kit, FeCl3 is present in sufficient amount that EDTA (used at the concentration as anti-coagulant in plasma/serum samples) will not interfere with the assay. One would need to use a lot of ...

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    Will these kits detect other isoforms of ascorbic acid besides the L-enantiomer (vitamin C)?

    Thank you for your inquiry. I just heard back from the testing laboratory that these kits cannot distinguish ascorbic acid isoforms. I am sorry that this information could not be more helpful, but please let us know if we can be of any other help.

    samples are frozen plasma with EDTA (not serum) about 3 samples of blood per patient (normal, before treatment, after treatment) 1. Can plasma samples be used (would plasma proteins interfere)? 2. If proteins interfere, can they be removed after thaw...

    Read More

    Thank you for contacting us. I have hear back from the ab with the following information to your questions: 1. Yes, you can use plasma samples – but for ab65656 we recommend removing the proteins (e.g. by using spin filter (10 kDa cutoff) (ab93349)...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"