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Ascorbic Acid Assay Kit (Biological Samples) (ab65656) provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Fe3+ is reduced to Fe2+ by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between OD=545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.
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Suitable for biological samples Range: 0.2 to 20 nmol of ascorbic acid
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference.
|Ascorbate Oxidase (lyophilized)||1 vial|
|Ascorbic Acid Probe||1 x 1ml|
|Ascorbic Acid Standard (Lyophilised)||1 vial|
|FeCl3 solution||1 x 1ml|
|Ferric Reducing Ascorbate Buffer||1 x 25ml|
Our Abpromise guarantee covers the use of ab65656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Standard curve : mean of duplicates (+/-SD) with background readings subtracted