Ascorbic Acid Assay Kit (Biological Samples) (ab65656)
- Product nameAscorbic Acid Assay Kit (Biological Samples)See all Ascorbic acid kits ...
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
- Assay typeQuantitative
- Sensitivity> 0.2 nmol/well
- Range0.2 nmol/well - 20 nmol/well
- Assay time0h 30m
- Product overview
Abcam's Ascorbic Acid Assay Kit (Biological Samples) provides a rapid, simple, and sensitive means of detecting ascorbic acid in biological samples such as serum and other body fluids, tissue and cell extracts, growth media and food products. In this assay, Fe3+ is reduced to Fe2+ by any antioxidants present. The ferrous iron is chelated with a colorimetric probe to produce a product with a strong absorbance band which can be monitored between 545-600 nm. The addition of ascorbate oxidase to parallel samples removes any ascorbate present leaving a background value which is subtracted from the total to give ascorbate content. The assay can detect 0.2 to 20 nmol of ascorbic acid in various samples.
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Suitable for biological samples
Range: 0.2 to 20 nmol of ascorbic acid
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent and an immune stimulant and is present in a wide variety of biological specimens. Due to the presence of a variety of other antioxidants in biological samples such as serum, most ascorbic acid assays show strong interference.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Ascorbate Oxidase (lyophilized) 1 vial Ascorbic Acid Probe 1 x 1ml Ascorbic Acid Standard (Lyophilised) 1 vial FeCl3 solution 1 x 1ml Ferric Reducing Ascorbate Buffer 1 x 25ml
- Research Areas
Our Abpromise guarantee covers the use of ab65656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Ascorbic Acid Assay Kit (Biological Samples) (ab65656)
This product has been referenced in:
- Dai D et al. Healing of saccular aneurysms following platinum coil embolization: lack of improved efficacy with vitamin C supplementation. J Neurointerv Surg N/A:N/A (2012). Functional Studies ; Rabbit . Read more (PubMed: 22914744) »