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Ascorbic Acid Assay Kit (ab65346)

Q&A (10)

Overview

  • Product nameAscorbic Acid Assay KitSee all Ascorbic acid kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • Assay typeQuantitative
  • Range
    0.1 nmol/well - 10 nmol/well
  • Assay time
    0h 5m
  • Product overview

    Abcam's Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceAscorbic Acid (C6H8O6) is an essential nutrient in human diets, and is necessary to maintain connective tissue and bone. The biologically active form of ascorbic acid is vitamic C, a water soluble vitamin which is the L-enantiomer form of ascorbic acid. Humans have lost the ability to synthesize ascorbic acid and must obtain in the diet. Ascorbic acid is an electron donor for enzymes involved in collagen hydroxylation, biosynthesis of carnitine and norepinephrine, tyrosine metabolism and amidatin of peptide hormones. The ability of vitamin C to donate electrons also makes it a potent water-soluble anti-oxidant that readily scavenges free radicals.

    • Applications

    Our Abpromise guarantee covers the use of ab65346 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Ascorbic Acid Assay Kit images

    • Functional Studies - Ascorbic Acid Assay Kit (ab65346)
      Functional Studies - Ascorbic Acid Assay Kit (ab65346)
      Ascorbic Acid Standard Curve.

    Protocols

    References for Ascorbic Acid Assay Kit (ab65346)

    ab65346 has not yet been referenced specifically in any publications.

    Publishing research using ab65346 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab65346 Submit a Question for ab65346

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    Should previously frozen samples be warmed to room temperature before use?
    For samples of 20% serum diluted in non-protein media, should ab65346 or ab65656 be used?

    Frozen samples should be thawed at room temperature, then kept on ice and used promptly in the assay (not warmed to room temperature). Ab65346 is recommended for your samples, since they are already diluted and will be further diluted during the assay. Ab...

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    I want to double-check the concentrations of the standards.

    Thank you for your inquiry.

    The lab was able to provide this information:

    1 mole/lt is 1 M.
    This standard is 20 µmole. You reconstitute it in 200 µl. That is 20 µmole/200 µl (or 100 µmole/ml which is ...

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    Can this kit be used with EDTA in theplasma samples?

    Thank you for calling Abcam earlier today.

    I have talked to the lab about whether the presence of EDTA in the samples would affect the kit and they say that it should be ok.

    Please let me know if you have any more questions.

    I'm trying to detect vit C in cell lysates.   My concern is that, if the determination of oxidized vitamin C by this kit is via a carbonyl group-specific color reaction, is it possible that cell lysates containing a high level of protein carbonyl groups w...

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    I understand your concern. Signal from pre-existing carbonyl groups, before the catalytic generation of  carbonyl from ascorbic acid, would be an issue with other samples types too, including serum and plasma. The background contributed by sample proteins...

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    My samples are in solutions with PVP, which contains many carbonyl groups. Will this interfere with the reactions? I know that vitamin C kits often utilize reactions on carbonyl groups. Also, I'm interested in determining the amounts of reduced an...

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    Thanks for your call this week and for your patience while I have been in touch with the lab about ab65346. This kit does utilize a reaction on carbonyl groups, so it will likely cross-react with the PVP though this has not been specifically tested...

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    Will these kits detect other isoforms of ascorbic acid besides the L-enantiomer (vitamin C)?

    Thank you for your inquiry. I just heard back from the testing laboratory that these kits cannot distinguish ascorbic acid isoforms. I am sorry that this information could not be more helpful, but please let us know if we can be of any other help.

    samples are frozen plasma with EDTA (not serum) about 3 samples of blood per patient (normal, before treatment, after treatment) 1. Can plasma samples be used (would plasma proteins interfere)? 2. If proteins interfere, can they be removed after thaw...

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    Thank you for contacting us. I have hear back from the ab with the following information to your questions: 1. Yes, you can use plasma samples – but for ab65656 we recommend removing the proteins (e.g. by using spin filter (10 kDa cutoff) (ab93349)...

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    I want to use plant samples with one of these kits, which may contain high concentrations of ascorbic acid. Would ab65656 be better for this as I see it's suitable for food products? Do you have a plant sample preparation protocol?

    Thank you for your inquiry. Of the two kits, ab65656 is more suitable. Theoretically ab65656 should be compatible with plant samples as long as the lysates are prepared in the assay buffer provided in the kit. Technically though, since we have not...

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    Can ab65346 or ab65656 be used on serum samples that have been frozen for several months? Is there any special preparation that should be done to avoid oxidation of the ascorbic acid?

    Thank you for contacting us. For serum samples, we recommend using ab65656. This kit produces more accurate results on samples with high protein content. The ascorbic acid will be more stable when the serum samples are deproteinated before storage b...

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    I have homogenized liver and serum samples that have been prepared in 5% TCA to deproteinate them (we were planning to use the old Ascorbic Acid Method based on Bessey, Lowry and Brock). Could we use these samples with your Ascorbic Acid Assay Kit (ab...

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    Thank you for your enquiry. We usually recommend ab65656 for serum samples, however both ab65346 and ab65656 should be fine with you samples since you have deproteinated with TCA. Simply use your sample as directed in the kit's protocol (link on datas...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"