Ascorbic Acid Assay Kit (ab65346)
- Product nameAscorbic Acid Assay KitSee all Ascorbic acid kits ...
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
- Assay typeQuantitative
- Range0.1 nmol/well - 10 nmol/well
- Assay time0h 5m
- Product overview
Abcam's Ascorbic Acid Assay Kit provides a rapid, simple, and sensitive means of detecting ascorbic acid in various biological samples. In this assay, our proprietary catalyst oxidizes ascorbic acid to produce a product that interacts with the ascorbic acid probe, generating color and fluorescence. Ascorbic acid can be easily determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods. The assay can detect 0.01-10 nmol of ascorbic acid per assay in various samples.
Visit our FAQs page for tips and troubleshooting.
Ascorbic Acid (Vitamin C) plays an important role in many biological processes. It is a potent anti-oxidant, anti-inflammatory, anti-viral agent, and an immune stimulant and is present is a wide variety of foods and biological specimens. It is important to be able to monitor ascorbic acid content in these different samples.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Ascorbic Acid Assay Buffer WM 1 x 25ml Ascorbic Acid Enzyme Mix (lyophilized) Green 1 vial Ascorbic Acid Probe (DMSO) Red 1 x 0.2ml Ascorbic Acid Standard (20 µmole) Yellow 1 vial Catalyst Blue 1 x 500µl
Our Abpromise guarantee covers the use of ab65346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Ascorbic Acid Assay Kit (ab65346)
ab65346 has not yet been referenced specifically in any publications.