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Abcam provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected colorimetrically (λ=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
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Asparaginase (EC 184.108.40.206) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs). The enzyme has been used to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products and to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). Metabolization of asparagine prevents acrylamide formation in fried foods (Maillard reaction). The enzyme's antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy.
|Asparaginase Assay Buffer||WM||1 x 25ml|
|Aspartate Enzyme Mix (lyophilized)||Green||1 vial|
|Aspartate Standard (100 mM)||Yellow||1 x 0.1ml|
|Conversion Mix (lyophilized)||Purple||1 vial|
|Positive Control (lyophilized)||Blue||1 vial|
|Probe (in DMSO)||Red||1 x 0.2ml|
|Substrate Mix (lyophilized)||Orange||1 vial|
Our Abpromise guarantee covers the use of ab107922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Aspartate measured in cell lysates after 10 min and 30 min incubation time showing quantity (nmol) per mL of tested sample.
Asparaginase measured in cell lysates showing quantity (mU) per mL of tested sample.
ab107922 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"