Asparaginase Activity Assay Kit (ab107922)
- Product nameAsparaginase Activity Assay Kit
- Tests100 x 1 assay
- Sample typeMilk, Other biological fluids, Cell Lysate
- Assay typeEnzyme activity
- Assay time0h 40m
- Product overview
Abcam provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected colorimetrically (λ=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
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Asparaginase (EC 22.214.171.124) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs). The enzyme has been used to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products and to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). Metabolization of asparagine prevents acrylamide formation in fried foods (Maillard reaction). The enzyme's antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
- Storage bufferPreservative: None
Components Identifier 100 tests Asparaginase Assay Buffer WM 1 x 25ml Aspartate Enzyme Mix (lyophilized) Green 1 vial Aspartate Standard (100 mM) Yellow 1 x 0.1ml Conversion Mix (lyophilized) Purple 1 vial Positive Control (lyophilized) Blue 1 vial Probe (in DMSO) Red 1 x 0.2ml Substrate Mix (lyophilized) Orange 1 vial
- Research Areas
Our Abpromise guarantee covers the use of ab107922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Asparaginase Activity Assay Kit (ab107922)
ab107922 has not yet been referenced specifically in any publications.