Overview

  • Product nameAsparaginase Activity Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Milk, Other biological fluids, Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    0h 40m
  • Product overview

    Abcam provides a simple, direct and automation-ready procedure for measuring asparaginase activity in biological samples. In the assay, Asparaginase hydrolyzes asparagine to generate aspartic acid, which can be detected colorimetrically (λ=570 nm) or fluorescently (Ex/Em = 535/590 nm) using a coupled enzymatic reaction.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Asparaginase (EC 3.5.1.1) is a homotetramer that catalyzes the hydrolysis of asparagine to aspartic acid and ammonia and exhibits about a 2-4% activity on glutamine and 5% on D-asparagine. Asparaginase does not occur naturally in humans but is found in bacteria, plants and many animals (e.g. guinea pigs). The enzyme has been used to reduce acrylamide, a suspected carcinogen, produced in fried starchy food products and to treat acute lymphoblastic leukemia (ALL) and some other hematopoietic neoplasms (e.g. multiple myeloma). Metabolization of asparagine prevents acrylamide formation in fried foods (Maillard reaction). The enzyme's antineoplastic effects are based on the inability of cancer cells (unlike healthy cells) to synthesize asparagine. However, the enzyme is not without some antigenicity and toxicity so it is very important to measure its activity in biological samples or monitor its activity during therapy.

  • Tested applicationsFunctional Studies more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab107922 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent dilution.

    Asparaginase Activity Assay Kit images

    • Examples of Standard curve obtained using ab107922

    Protocols

    References for Asparaginase Activity Assay Kit (ab107922)

    ab107922 has not yet been referenced specifically in any publications.

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    It is ideal to collect serum from fresh blood samples, aliquot them and store at -80 degrees C for short periods of time (a couple of months) for this assay. EDTA is usually avoided in enzymatic assays because of its chelating properties.
    I a...

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    This assay works on the principle of the Asparaginase present in your samples to hydrolyze asparagine to produce aspartic acid. This aspartic acid has to be further converted to another intermediate which finally reacts with the probe on order to gener...

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    Thank you for your enquiry and interest in our products.
    This assay kit (ab107922) works on the principle of the Asparaginase present in your samples to hydrolyze Asparagine to produce Aspartic acid. This Aspartic acid has to be further converted ...

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    we are not so certain that whole blood can be used as a sample type with this kit.
    The red colour of the blood will interfere significantly with the final reading.
    We would recommend using plasma/serum samples for this purpose. Plasma/serum ca...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"