Recombinant
RabMAb

Anti-Aspartate Aminotransferase antibody [EPR12145] (Alexa Fluor® 488) (ab201269)

Overview

  • Product name
    Anti-Aspartate Aminotransferase antibody [EPR12145] (Alexa Fluor® 488)
    See all Aspartate Aminotransferase primary antibodies
  • Description
    Rabbit monoclonal [EPR12145] to Aspartate Aminotransferase (Alexa Fluor® 488)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Aspartate Aminotransferase aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P17174

  • Positive control
    • ICC/IF: MCF7 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in MCF7 cells fixed with 100% methanol (5 min)

Target

  • Function
    Plays a key role in amino acid metabolism.
  • Sequence similarities
    Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AATC_HUMAN antibody
    • Aspartate aminotransferase 1 antibody
    • Aspartate aminotransferase antibody
    • Aspartate aminotransferase cytoplasmic antibody
    • Aspartate aminotransferase cytosolic antibody
    • ASTQTL1 antibody
    • cAspAT antibody
    • cCAT antibody
    • cysteine aminotransferase, cytoplasmic antibody
    • cysteine transaminase, cytoplasmic antibody
    • cytoplasmic antibody
    • ec 2.6.1.1 antibody
    • GIG 18 antibody
    • GIG18 antibody
    • Glutamate oxaloacetate transaminase 1 antibody
    • Glutamate oxaloacetate transaminase soluble antibody
    • Glutamic oxaloacetic transaminase 1 soluble antibody
    • GOT 1 antibody
    • GOT1 antibody
    • Growth inhibiting protein 18 antibody
    • SGOT antibody
    • Transaminase A antibody
    see all

Images

  • ab201269 staining Aspartate Aminotransferrase in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201269 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue, with nucleolar fixation artefact).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab201269 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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