Anti-Aspartate Aminotransferase antibody (ab98228)

Overview

  • Product nameAnti-Aspartate Aminotransferase antibody
    See all Aspartate Aminotransferase primary antibodies
  • Description
    Rabbit polyclonal to Aspartate Aminotransferase
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Pig, Saccharomyces cerevisiae, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 (MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV) of Human Aspartate Aminotransferase (NP_002070).

  • Positive control
    • NCI-H226 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab98228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

Anti-Aspartate Aminotransferase antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Aspartate Aminotransferase with ab98228 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Aspartate Aminotransferase. Right - Merge.
  • Anti-Aspartate Aminotransferase antibody (ab98228) at 1 µg/ml (in 5% skim milk / PBS buffer) + NCI-H226 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Gel concentration: 12%

References for Anti-Aspartate Aminotransferase antibody (ab98228)

ab98228 has not yet been referenced specifically in any publications.

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