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Anti-Aspartate Aminotransferase antibody (ab98228)

Submit an Abreview Q&A (2)

Overview

  • Product nameAnti-Aspartate Aminotransferase antibodySee all Aspartate Aminotransferase primary antibodies ...
  • Description
    Rabbit polyclonal to Aspartate Aminotransferase
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 (MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV) of Human Aspartate Aminotransferase (NP_002070).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlNCI-H226 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab98228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Alternative names
      AATC_HUMAN antibodyAspartate aminotransferase 1 antibodyAspartate aminotransferase antibody
      Aspartate aminotransferase cytoplasmic antibodyAspartate aminotransferase cytosolic antibodycytoplasmic antibodyec 2.6.1.1 antibodyGIG 18 antibodyGIG18 antibodyGlutamate oxaloacetate transaminase 1 antibodyGlutamate oxaloacetate transaminase soluble antibodyGlutamic oxaloacetic transaminase 1 soluble antibodyGOT 1 antibodyGOT1 antibodyGrowth inhibiting protein 18 antibodySGOT antibodyTransaminase a antibody
    see all

Anti-Aspartate Aminotransferase antibody images

  • Western blot - Aspartate Aminotransferase antibody (ab98228)
    Western blot - Aspartate Aminotransferase antibody (ab98228)
    Anti-Aspartate Aminotransferase antibody (ab98228) at 1 µg/ml (in 5% skim milk / PBS buffer) + NCI-H226 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa

References for Anti-Aspartate Aminotransferase antibody (ab98228)

ab98228 has not yet been referenced specifically in any publications.

Publishing research using ab98228 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab98228 Submit a Question for ab98228

Displaying 1 - 2 of 2 results for Abreviews and Q&A

10
Votes: Highest First

Thank you for your quick reply. Indeed there are two isoforms of the
Aspartate aminotransferase (also referred to as Glutamate oxaloacetate
transaminase), one being localized in the cytosol, namly GOT1
(http://www.uniprot.org/uniprot/P17174...

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Thank you for your email. My colleague has passed this inquiry to me as she is busy today.

I have checked the sequence similarity between the two forms (http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clu...

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Dear Sir or Madam, I am searching for two specific antibodies against the cytosolic form (GOT1) and the mitochondrial form of the human aspartate aminotransferase. I have already tested antibodies, which claimed to be specific, from another company, howev...

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Thank you for contacting us.

We have indeed three antibodies which target GOT1. However, after checking the SwissProt database, I cannot find any information about different isoforms. Could you please go into more detail why the other antibodie...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"