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- Secondary antibodies
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Product name
Anti-Asporin antibody
See all Asporin products (3) ...
Description
Goat polyclonal to Asporin
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: C-IHENKVKKIQKDT, corresponding to Internal sequence amino acids 182-194 of Human Asporin
C-IHENKVKKIQKDT
Positive control
Human Bone Marrow lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. PubMed: 20541802
ICC/IF: Use at an assay dependent dilution.
Target
Relevance
ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin. Asporin is expressed in a variety of human tissues with higher levels in osteoarthritic articular cartilage, aorta, uterus, heart and liver. The function of Asporin is unclear, but available data indicates a collagen binding capacity.
Cellular localization
Secreted, extracellular space; extracellular matrix.
Alternative names
- ASPN antibody
- ASPN protein antibody
- asporin (LRR class 1) antibody
see all
Database links
- Entrez Gene: 54829 Human
- Entrez Gene: 66695 Mouse
- Omim: 608135 Human
- SwissProt: Q9BXN1 Human
- SwissProt: Q5TBF2 Human
- SwissProt: Q5TBF3 Human
- SwissProt: Q6P528 Human
- SwissProt: Q05CQ6 Human
see all
Anti-Asporin antibody images:
Western blot - Asporin antibody (ab31303)
Anti-Asporin antibody (ab31303) at 0.3 µg/ml + Human Bone Marrow lysate (35µg protein in RIPA buffer)
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence - Asporin antibody (ab31303)
Ab31303 staining of ATDC5 cells (Panels A and C) and DAPI (panels B and D). Image shows staining of cytoplasm and on the cell surface. Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.
References for Anti-Asporin antibody (ab31303)
This product has been referenced in:
- Hurng JM et al. Discontinuities in the human bone-PDL-cementum complex. Biomaterials 32:7106-17 (2011). ICC/IF; Human.Read more (PubMed: 21774982) »
- Ho SP et al. The biomechanical characteristics of the bone-periodontal ligament-cementum complex. Biomaterials 31:6635-46 (2010). IHC-P; Human.Read more (PubMed: 20541802) »
See all 2 publications for this product
Publishing research using ab31303? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"