Astrocytomas-low grade antibody [J1-31] (ab80189)

Product Name 

Astrocytomas-low grade antibody [J1-31]

Product type 

Primary antibodies

Description 

Mouse monoclonal [J1-31] to Astrocytomas-low grade

Immunogen 

Tissue / cell preparation - Human cerebral white matter from a multiple sclerosis patient

Reacts with 
(species key)

Hu, Ms, Rat, Gpig

Specificity 

ab80189 recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS). ab80189 has recently been found to be a specific marker for low grade astrocytoma in human brain tissue and is able to distinguish between low grade astrocytoma and normal reactive gliosis.

Tested applications 
(see key)

ICC, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: Reducing conditions, recognizes a 30kDa protein. Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cancer >> Tumor biomarkers >> Other
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Type Marker >> Glia marker >> Astrocyte marker

Relevance 

An astrocytoma is a form of glioma tumor which originates in the star-shaped brain cells called astrocytes. There is a four-tiered histologic grading guideline for astrocytomas with the low grade astrocytomas (I & II) being among the least common of all reported brain tumors, less than 6%.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

J1-31

Isotype 

IgM

Purity 

Ascites

Storage buffer 

Preservative: None
Constituents: Ascites

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

References for Astrocytomas-low grade antibody [J1-31] (ab80189)

  This clone has been used in:

Predy R et al. A new protein (J1-31 antigen, 30 kD) is expressed by astrocytes, Mller glia and ependyma. Biosci Rep 7:491-502 (1987). PubMed: 3427214

If you publish research using ab80189 please let us know so that we can cite the reference on this datasheet.

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