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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-AtRAC3 antibody
Description
Rabbit polyclonal to AtRAC3
Specificity
Sequence used for immunogen is identical in AtRac 1, 2, 4, 5, 6, 8, 10 and 11 and in RAC/Rop of many plant species. It is highly conserved (single amino acid substitution)in AtRac7 and AtRac9
Tested applications
Cross reactivity
Reacts with
Arabidopsis thaliana
Immunogen
Synthetic peptide: TAGQEDYNRLRPLSYRGAD conjugated to KLH, corresponding to N terminal amino acids 61-79 of Arabidopsis thaliana RAC3
TAGQEDYNRL RPLSYRGAD
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Plant related targets
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Western blot - AtRAC3 antibody (ab50678)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.25 - 0.5 µg/ml.Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Target
Relevance
Plants contain a unique Rho GTPase subfamily designated as RAC or Rop (Rho from plants), subdivided into two major subgroups according to whether they are prenylated or palmitoylated. RAC/Rop proteins regulate a wide array of cellular processes. They induce reorganization of actin, and have been implicated in changes in polar growth patterns, inducing Ca2+ influx at the tip in germinating pollen tubes, regulating various hormone-mediated plant signalling and developmental processes, the production of reactive oxygen species and modulating the pathogen-induced hypersensitive response (HR). In Arabidopsis, 11 different RAC/Rop GTPases have been identified so far, but the function of the individual RACs is not yet fully understood.
Cellular localization
Cytoplasmic and Cell Membrane; peripheral membrane protein. Note: Associated with the membrane when activated.
Alternative names
- ARAC3 antibody
- ATROP6 antibody
- GTPase protein ROP6 antibody
see all
Database links
- Entrez Gene: 829654 Arabidopsis thaliana
- SwissProt: Q38912 Arabidopsis thaliana
Anti-AtRAC3 antibody images:
Western blot - AtRAC3 antibody (ab50678)
All lanes : Anti-AtRAC3 antibody (ab50678) at 1/2000 dilution
Lane 1 : E. coli extract expressing AtRAC3 (10µg)
Lane 2 : E. coli extract expressing AtRAC3 (5µg)
Lane 3 : E. coli extract expressing AtRAC3 (2.5µg)
Lane 4 : E. coli extract expressing AtRAC3 (1.2µg)
Lane 5 : E. coli extract expressing AtRAC3 (0.6µg)
Lane 6 : E. coli extract expressing AtRAC3 (5mg) with immunizing peptide at 10 µg/ml
Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
developed using the ECL technique
Predicted band size : 22 kDa
Observed band size : 22 kDa
References for Anti-AtRAC3 antibody (ab50678)
ab50678 has not yet been referenced specifically in any publications.
Publishing research using ab50678? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"