AtRAC3 antibody (ab50678)

Product Name 

AtRAC3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to AtRAC3

Immunogen 

Synthetic peptide: TAGQEDYNRLRPLSYRGAD conjugated to KLH, corresponding to N terminal amino acids 61-79 of Arabidopsis thaliana RAC3
BLAST 'TAGQEDYNRLRPLSYRGAD' with or

Reacts with 
(species key)

At

Specificity 

Sequence used for immunogen is identical in AtRac 1, 2, 4, 5, 6, 8, 10 and 11 and in RAC/Rop of many plant species. It is highly conserved (single amino acid substitution)in AtRac7 and AtRac9

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic and Cell Membrane; peripheral membrane protein. Note: Associated with the membrane when activated.

Research areas 

Cell Biology >> Other Antibodies >> Plant related targets
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family

Relevance 

Plants contain a unique Rho GTPase subfamily designated as RAC or Rop (Rho from plants), subdivided into two major subgroups according to whether they are prenylated or palmitoylated. RAC/Rop proteins regulate a wide array of cellular processes. They induce reorganization of actin, and have been implicated in changes in polar growth patterns, inducing Ca2+ influx at the tip in germinating pollen tubes, regulating various hormone-mediated plant signalling and developmental processes, the production of reactive oxygen species and modulating the pathogen-induced hypersensitive response (HR). In Arabidopsis, 11 different RAC/Rop GTPases have been identified so far, but the function of the individual RACs is not yet fully understood.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.600 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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