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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-AtRabA4 antibody
Description
Rabbit polyclonal to AtRabA4
Specificity
ab55896 specifically recognizes recombinant AtRabA4b fusion protein
Tested applications
Cross reactivity
Reacts with
Arabidopsis thaliana, Soybean
Immunogen
Synthetic peptide: GKKILIPGSGQEIPAKTST conjugated to KLH, corresponding to C terminal amino acids 201-219 of Arabidopsis thaliana AtRabA4
GKKILIPGSG QEIPAKTST
Positive control
Whole cell extract of E. coli expressing AtRabA4b fusion protein
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by
centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Plant related targets
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa. Staining of the AtRabA4b band in immunoblotting is specifically inhibited by the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Rab GTPases belong to the superfamily of small GTP binding proteins that include Ras, Rho, Arf and Ran GTPases. The Rab GTPases are involved in the regulation of diverse eukaryotic cellular processes such as cytoskeletal assembly and organization, cellproliferation and intracellular membrane trafficking. In eukaryotic cells Rab GTPases regulate membrane trafficking events associated with distinct compartments. The type of subcellular compartment with which Rab GTPase is associated can be predicted based on sequence similarity. The Arabidopsis genome contains a total of 93 small GTPase genes, including 57 members of the Rab family. Arabidopsis thaliana Rab GTPase RabA4b is highly similar to AtRab11. AtRabA4b labels a novel compartment that accumulates at the tips of expanding root hair cells. It has been suggested that in A. thaliana, the RabA4b GTPase regulates membrane trafficking steps involved in the polarized deposition of cell wall components in tip-growing root hair cells. AtRabA4b has been shown to label a novel, trans-Golgi network compartment displaying a developmentally regulated polar distribution in growing A. thaliana root hair cells. GTP bound AtRabA4b selectively recruits the plant phosphatidylinositol-4- kinase PI-4Kb1, but not other members of PI-4K families.
Database links
- Entrez Gene: 830160 Arabidopsis thaliana
- SwissProt: Q38923 Arabidopsis thaliana
- Unigene: 1649 Arabidopsis thaliana
References for Anti-AtRabA4 antibody (ab55896)
ab55896 has not yet been referenced specifically in any publications.
Publishing research using ab55896? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"