AtRabA4 antibody (ab55896)

Product Name 

AtRabA4 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to AtRabA4

Immunogen 

Synthetic peptide: GKKILIPGSGQEIPAKTST conjugated to KLH, corresponding to C terminal amino acids 201-219 of Arabidopsis thaliana AtRabA4
BLAST 'GKKILIPGSGQEIPAKTST' with or

Reacts with 
(species key)

At, Soy

Specificity 

ab55896 specifically recognizes recombinant AtRabA4b fusion protein

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa. Staining of the AtRabA4b band in immunoblotting is specifically inhibited by the immunizing peptide.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole cell extract of E. coli expressing AtRabA4b fusion protein

Research areas 

Cell Biology >> Other Antibodies >> Plant related targets
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family

Relevance 

Rab GTPases belong to the superfamily of small GTP binding proteins that include Ras, Rho, Arf and Ran GTPases. The Rab GTPases are involved in the regulation of diverse eukaryotic cellular processes such as cytoskeletal assembly and organization, cellproliferation and intracellular membrane trafficking. In eukaryotic cells Rab GTPases regulate membrane trafficking
events associated with distinct compartments. The type of subcellular compartment with which Rab GTPase is associated can be predicted based on sequence similarity.
The Arabidopsis genome contains a total of 93 small GTPase genes, including 57 members of the Rab family. Arabidopsis thaliana Rab GTPase RabA4b is highly similar to AtRab11. AtRabA4b labels a novel compartment that accumulates at the tips of expanding root hair cells. It has been suggested that in A. thaliana, the RabA4b GTPase regulates membrane trafficking steps involved in the polarized deposition of cell wall components in tip-growing root hair cells. AtRabA4b has been shown to label a novel, trans-Golgi network compartment displaying a developmentally regulated polar distribution in growing A. thaliana root hair cells. GTP bound AtRabA4b selectively recruits the plant phosphatidylinositol-4- kinase PI-4Kb1, but not other members of PI-4K families.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

If slight turbidity occurs upon prolonged storage, clarify the solution by
centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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