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Product Name
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AtT-20/D16v-F2 (Mouse pituitary epithelial-like tumor cell line) Nuclear Lysate
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Product type
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Lysates
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Description
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AtT-20/D16v-F2 (Mouse pituitary epithelial-like tumor cell line) Nuclear Lysate
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Application notes
(see key)
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Recommended dilutions
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Mouse >> Other
Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Mouse >> Endocrine
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Relevance
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atT-20 cells are corticotroph and are derived from a neuroendocrine tumor, which endogenously expresses two distinct somatostatin receptor subtypes (SRIF). They are used in transfection studies to investigate endocrine and exocrine secretory pathways, in particular ACTH (adrenocorticotropic hormone) and beta-endorphin.
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Storage buffer
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Preservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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Form
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Liquid
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Concentration
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6.340 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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We recommend aliquoting the extracts into single use fractions and then
storing.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this AtT-20/D16v-F2 (Mouse pituitary epithelial-like tumor cell line) Nuclear Lysate is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to AtT-20/D16v-F2
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