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Products:Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Mouse >> Endocrine
Overview
Product name
AtT-20/D16v-F2 (Mouse pituitary epithelial-like tumor cell line) Nuclear Lysate
General notes
We recommend aliquoting the extracts into single use fractions and then
storing.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
Concentration
Concentration information loading...
Research areas
Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Mouse >> Other
Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Mouse >> Endocrine
Background
atT-20 cells are corticotroph and are derived from a neuroendocrine tumor, which endogenously expresses two distinct somatostatin receptor subtypes (SRIF). They are used in transfection studies to investigate endocrine and exocrine secretory pathways, in particular ACTH (adrenocorticotropic hormone) and beta-endorphin.
Applications
Application notes
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for AtT-20/D16v-F2 (Mouse pituitary epithelial-like tumor cell line) Nuclear Lysate (ab14636)
ab14636 has not yet been referenced specifically in any publications.
Publishing research using ab14636? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"