Anti-Ataxin 1 antibody [N65/37] (ab186393)
Key features and details
- Mouse monoclonal [N65/37] to Ataxin 1
- Suitable for: WB, ICC
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Ataxin 1 antibody [N65/37]
See all Ataxin 1 primary antibodies -
Description
Mouse monoclonal [N65/37] to Ataxin 1 -
Host species
Mouse -
Specificity
No cross-reactivity against phosphor S751-Ataxin 1. Minimal cross-reactivity against S751A mutant of Ataxin 1 by ELISA and immunofluorescence and negative by immunoblot. -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide corresponding to Mouse Ataxin 1 aa 700 to the C-terminus (C terminal).
Database link: P54254 -
Positive control
- COS cells transiently transfected with Ataxin 1.
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General notes
The clone number has been updated from S65-37 to N65/37, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N65/37 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 84 kDa.
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ICC |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Predicted molecular weight: 84 kDa. |
ICC
Use at an assay dependent concentration. |
Target
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Function
Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function. -
Tissue specificity
Widely expressed throughout the body. -
Involvement in disease
Defects in ATXN1 are the cause of spinocerebellar ataxia type 1 (SCA1) [MIM:164400]; also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA1 is caused by expansion of a CAG repeat in the coding region of ATXN1. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
Sequence similarities
Belongs to the ATXN1 family.
Contains 1 AXH domain. -
Domain
The AXH domain is required for interaction with CIC. -
Post-translational
modificationsPhosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract. -
Cellular localization
Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6310 Human
- Omim: 601556 Human
- SwissProt: P54253 Human
- Unigene: 434961 Human
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Alternative names
- alternative ataxin1 antibody
- Ataxin-1 antibody
- ATX1 antibody
see all
Images
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Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.
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Western Blot analysis of Monkey COS-1 cells transfected with Ataxin- 1 showing detection of ~85 kDa Ataxin 1 protein using Mouse Anti-Ataxin 1 Monoclonal Antibody, Clone S65-37. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS-1 cells transfected with Ataxin- 1. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Ataxin 1 Monoclonal Antibody at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~84 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab186393 has not yet been referenced specifically in any publications.