Anti-Ataxin 1 antibody (ab114045)
Key features and details
- Rabbit polyclonal to Ataxin 1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ataxin 1 antibody
See all Ataxin 1 primary antibodies -
Description
Rabbit polyclonal to Ataxin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide, corresponding to a region between amino acids 350-400 of Human Ataxin 1 (NP_000323.2).
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Positive control
- HeLa whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 10 µg/mg of lysate.
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Notes |
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IP
Use at 10 µg/mg of lysate. |
Target
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Function
Binds RNA in vitro. May be involved in RNA metabolism. The expansion of the polyglutamine tract may alter this function. -
Tissue specificity
Widely expressed throughout the body. -
Involvement in disease
Defects in ATXN1 are the cause of spinocerebellar ataxia type 1 (SCA1) [MIM:164400]; also known as olivopontocerebellar atrophy I (OPCA I or OPCA1). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to cerebellum degeneration with variable involvement of the brainstem and spinal cord. SCA1 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA1 is caused by expansion of a CAG repeat in the coding region of ATXN1. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
Sequence similarities
Belongs to the ATXN1 family.
Contains 1 AXH domain. -
Domain
The AXH domain is required for interaction with CIC. -
Post-translational
modificationsPhosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract. -
Cellular localization
Cytoplasm. Nucleus. Colocalizes with USP7 in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6310 Human
- Omim: 601556 Human
- SwissProt: P54253 Human
- Unigene: 434961 Human
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Alternative names
- alternative ataxin1 antibody
- Ataxin-1 antibody
- ATX1 antibody
see all
Images
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Detection of Ataxin 1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114045 at 10 µg/mg lysate for IP. An anti-Ataxin 1 antibody which recognizes a downstream epitope was used at 1 µg/ml for subsequent western blot detection. Detection: Chemiluminescence with exposure time of 3 seconds.
Predicted band size : 87 kDa.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab114045 has not yet been referenced specifically in any publications.