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Product name
Anti-Aurora A antibody
See all Aurora A products (21) ...
Description
Rabbit polyclonal to Aurora A
Tested applications
ICC/IF, IHC-P, WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Serine/Threonine Kinase 15 encoded within exon 5 (LocusLink ID 8465)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. Used at a dilution of 1/1000 for 1 hr on HeLa cells (see Abreview).
IHC-P: 1/250 - 1/1000.
WB: 1/4000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast, pancreas and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly after.
IP: Use a concentration of 2 - 10 µg/ml.
Target
Function
Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
Tissue specificity
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
Target information above from: UniProt accessionO14965
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
see all
Database links
- Entrez Gene: 6790 Human
- Omim: 603072 Human
- SwissProt: O14965 Human
- Unigene: 250822 Human
Anti-Aurora A antibody images:
Immunoprecipitation - Aurora A antibody (ab1287)
Immunoprecipitation of Human Aurora A.
35S-Met labeled whole cell lysate from cells transfected with a human Aurora A expression construct. ab1287 was used at 4 µg/ml for IP. Detection: Autoradiography.
Immunohistochemistry (Paraffin-embedded sections) - Aurora A antibody (ab1287)
ab1287 (1/250) detecting Aurora A in paraffin embedded Human pancreatic tumour tissue sections. Detection: DAB staining;
Immunocytochemistry/ Immunofluorescence - Aurora A antibody (ab1287)
ab1287 at a 1/1000 dilution staining synchronised HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 1% serum prior to incubation with the primary antibody for 1 hour. A Cy5 conjugated donkey anti-rabbit antibody was used as the secondary. The image shows a DAPI panel, Aurora-A panel, and merge panel of DAPI (blue), microtubules (red) and Aurora-A (green).
This image is courtesy of an Abreview submitted by Mr Michael Porter
Western blot - Aurora A antibody (ab1287)
Anti-Aurora A antibody (ab1287) at 1 µg/ml +
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 48 kDa
Observed band size : 48 kDa
Additional bands at : 20 kDa,25 kDa,28 kDa,37 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Aurora A antibody (ab1287)
This product has been referenced in:
- Keppner S et al. Biological impact of freezing Plk1 in its inactive conformation in cancer cells. Cell Cycle 9:761-73 (2010). IP; Human.Read more (PubMed: 20139717) »
- Cheung CH et al. BPR1K653, a Novel Aurora Kinase Inhibitor, Exhibits Potent Anti-Proliferative Activity in MDR1 (P-gp170)-Mediated Multidrug-Resistant Cancer Cells. PLoS One 6:e23485 (2011). WB; Human.Read more (PubMed: 21887256) »
See all 11 publications for this product
Publishing research using ab1287? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"