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Product name
Anti-Aurora A antibody
See all Aurora A products (21) ...
Description
Rabbit polyclonal to Aurora A
Specificity
ab12875 detects a single clean band of just below 50kD in Jurkat and Hela cell lysates. This band corresponds to the size of Aurora A seen using other antibodies.
Tested applications
IHC-P, WB, IF, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Aurora A.
(Peptide available as ab130 08.)
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IF
Application notes
ICC: 1/500.
IF: 1/500.
IHC-P: 1/15 - 1/20. Antigen retrieval was performed using either Sodium Citrate or Tris/EDTA before commencing with the IHC staining protocol. Customer feedback suggests that a 1/15 dilution of ab12875 is recommended when using the sodium citrate antigen retreival method and a 1/20 dilution is recommended when using the Tris/EDTA antigen retrieval method.
WB: 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
Tissue specificity
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
Target information above from: UniProt accessionO14965
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
see all
Database links
- Entrez Gene: 6790 Human
- Omim: 603072 Human
- SwissProt: O14965 Human
- Unigene: 250822 Human
Anti-Aurora A antibody images:
Western blot - Aurora A antibody (ab12875)
All lanes : Anti-Aurora A antibody (ab12875) at 1/500 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor 680 Goat anti Rabbit IgG at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 46 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry - Aurora A antibody (ab12875)
Metaphase HeLa cells stained with ab12875 and counterstained with DAPI. The antibody shows the localisation of Aurora A to the centrosome during metaphase. 100x magnification.
Panel 1: DNA stained with DAPI.
Panel 2: Cells stained with ab12875 (1/500 dilution).
Panel 3: DAPI staining is shown in red and ab12875 in green.
This image was submitted as part of a review by Kirk McManus, University of British Columbia
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Aurora A antibody (ab12875)
The image shows staining of formalin fixed paraffin embedded human tonsil sections with ab12875 (1/20 working dilution). Clear nuclear staining can be seen.
References for Anti-Aurora A antibody (ab12875)
This product has been referenced in:
- Morita E et al. Human ESCRT-III and VPS4 proteins are required for centrosome and spindle maintenance. Proc Natl Acad Sci U S A 107:12889-94 (2010). ICC/IF; Human.Read more (PubMed: 20616062) »
- Bird AW & Hyman AA Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A. J Cell Biol 182:289-300 (2008). WB; Human.Read more (PubMed: 18663142) »
See all 3 publications for this product
Publishing research using ab12875? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"