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Synthetic non-phosphopeptide derived from human Aurora A around the phosphorylation site of threonine 288 (R-T-TP-L-C).
Our Abpromise guarantee covers the use of ab61114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 µg/ml.|
|ICC/IF||1/500. (see Abreview)|
|WB||1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).|
ab61114 staining Aurora A in Human Fibroblast cells by Immunocytochemistry/Immunofluorescence. Cells were PFA-fixed and permeabilized in methanol for 30 minutes at -20°C prior to blocking in 20% FCS for 30 minutes at room temperature. The primary antibody was diluted 1/500 with the blocking buffer and incubated with the sample for 1 hour. The secondary antibody was a Alexa Fluor 594® conjugated Goat anti-Mouse polyclonal, diluted 1/1000.
DAPI was used for the nuclear counterstain.
ab61114 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"