Anti-Aurora A antibody (ab61114)

Overview

  • Product nameAnti-Aurora A antibodySee all Aurora A primary antibodies ...
  • Description
    Rabbit polyclonal to Aurora A
  • Specificityab61114 detects endogenous levels of total Aurora A protein.
  • Tested applicationsIHC-P, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human Aurora A around the phosphorylation site of threonine 288 (R-T-TP-L-C).

  • Positive control
    • extracts from 293 cells treated with serum (20%, 15mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab61114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 µg/ml.
ICC/IF 1/500. (see Abreview)
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).

Target

  • FunctionContributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
  • Tissue specificityHighly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIK antibody
    • ARK-1 antibody
    • ARK1 antibody
    • AURA antibody
    • AURKA antibody
    • Aurora 2 antibody
    • Aurora A antibody
    • Aurora Family Kinase 1 antibody
    • aurora kinase A antibody
    • Aurora Related Kinase 1 antibody
    • Aurora-related kinase 1 antibody
    • Aurora/IPL1 Like Kinase antibody
    • Aurora/IPL1 Related Kinase 1 antibody
    • Aurora/IPL1-related kinase 1 antibody
    • AURORA2 antibody
    • AYK1 antibody
    • BRAK antibody
    • Breast Tumor Amplified Kinase antibody
    • Breast tumor-amplified kinase antibody
    • BTAK antibody
    • hARK1 antibody
    • hARK1 antibody
    • IAK antibody
    • IPL1 Aurora Related Kinase 1 antibody
    • IPL1 Related Kinase antibody
    • MGC34538 antibody
    • OTTHUMP00000031340 antibody
    • OTTHUMP00000031341 antibody
    • OTTHUMP00000031342 antibody
    • OTTHUMP00000031343 antibody
    • OTTHUMP00000031344 antibody
    • OTTHUMP00000031345 antibody
    • OTTHUMP00000166071 antibody
    • OTTHUMP00000166072 antibody
    • PPP1R47 antibody
    • Protein phosphatase 1, regulatory subunit 47 antibody
    • Serine/threonine kinase 15 antibody
    • Serine/threonine kinase 6 antibody
    • Serine/threonine kinase antibody
    • Serine/threonine-protein kinase 15 antibody
    • Serine/threonine-protein kinase 6 antibody
    • Serine/threonine-protein kinase aurora-A antibody
    • STK15 antibody
    • STK6 antibody
    • STK6_HUMAN antibody
    • STK7 antibody
    see all

Anti-Aurora A antibody images

  • All lanes : Anti-Aurora A antibody (ab61114) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with serum (20%, 15mins)
    Lane 2 : Extracts from 293 cells treated with serum (20%, 15mins) with immunising Aurora A peptide


    Predicted band size : 46 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • ab61114 staining Aurora A in human ovarian carcinoma.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab61114 staining Aurora A in Human Fibroblast cells by Immunocytochemistry/Immunoflourescence. Cells were PFA-fixed and permeabilized in methanol for 30 minutes at -20°C prior to blocking in 20% FCS for 30 minutes at room temperature. The primary antibody was diluted 1/500 with the blocking buffer and incubated with the sample for 1 hour. The secondary antibody was a Alexa Fluor 594®-conjugated Goat anti-Mouse polyclonal, diluted 1/1000.
    DAPI was used for the nuclear counterstain.

    See Abreview

References for Anti-Aurora A antibody (ab61114)

ab61114 has not yet been referenced specifically in any publications.

Product Wall


I am very pleased to hear you would like to accept our offer and test ab61114 in Chlamy reinhardtii. This code will give you $*** off your next order before the expiration date. To redeem this offer, please submit an Abreview for Chlamy reinhardti...

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Thank you for your reply.

I apologize for the confusion, there was a difference in the numerical order of amino acids listed in the alignment I was performing. You are correct, ab115883 should not be chosen as the immunogen sequence does not o...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Fibroblasts)
Specification Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - Methanol, 30 min at -20°C
Blocking step FCS as blocking agent for 30 minute(s) · Concentration: 20%
Username

Dr. Ioannis Gavvovidis

Verified customer

Submitted Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"