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Product name
Anti-Aurora A antibody
See all Aurora A products (21) ...
Description
Goat polyclonal to Aurora A
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: QNKESASKQS, corresponding to C terminal amino acids 394-403 of Human Aurora A.
C-QNKESASKQS
Positive control
Jurkat and HeLa cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).
ELISA
ELISA: 1/128000.ELISA: 1/128000.
Target
Function
Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
Tissue specificity
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
Target information above from: UniProt accessionO14965
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIK antibody
- ARK-1 antibody
- ARK1 antibody
see all
Database links
- Entrez Gene: 6790 Human
- Omim: 603072 Human
- SwissProt: O14965 Human
- Unigene: 250822 Human
Anti-Aurora A antibody images:
Immunocytochemistry/ Immunofluorescence - Aurora A antibody (ab9689)
Prometaphase HeLa cells were fixed in 100% methanol for 6 minutes at -20°C. The cells were washed 3 times in PBS and incubated with ab9689 (1µg/ml) for 1 hour at room temperature. The panel shows the cells stained with DAPI (blue) and ab9689 (green). The final image in the panel shows the merged images. 100x magnification.
Roberto Giambruno and Giulia Guarguaglini
Western blot - Aurora A antibody (ab9689)
Anti-Aurora A antibody (ab9689) at 0.1 µg/ml + Jurkat lysate at 35 µg
Predicted band size : 46 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-Aurora A antibody (ab9689)
ab9689 has not yet been referenced specifically in any publications.
Publishing research using ab9689? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"