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Western blot - Aurora A (phospho T288) antibody (ab18318)
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- Aurora A (phospho T288) antibody (ab18318)
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Immunocytochemistry/ Immunofluorescence - Aurora A (phospho T288) antibody (ab18318)
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Product Name
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Aurora A (phospho T288) antibody
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See all Aurora A antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Aurora A (phospho T288)
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Immunogen
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Synthetic peptide: SRRTTPLCGT, corresponding to amino acids 284-292 of Human Aurora A
BLAST 'SRRTTPLCGT' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody has been shown to mildly react with Aurora B if excess antibody is applied (cross-reactivity with Aurora B can be observed in immunofluorescence)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/500 - 1/1000.
WB: 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). It is strongly recommended that immunoprecipitation with a pan-specific Aurora antibody be carried out before Western blotting with the phospho-specific antibody to increase signal. Weak non specific bands can be sometimes observed above the band of interrest. A large non specific band is also present just above 32kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Nocadozole-arrested HeLa cell lysate immunoprecipitate with a pan-Aurora A monoclonal antibody (clone 35C1)(ab13824)
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Cellular localization
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Localizes on centrosomes in interphase cells and at each spindle pole in mitosis
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Research areas
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Cancer >> Tumor biomarkers >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Relevance
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Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol. pH 7.2 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Hela cells were synchronized to M phase using nocadazole (18 hrs treatment). Mitotic shake-off cells were used to prepare extracts. For a 10 cm2 dish (approximately 80% confluent, about 60% in M-phase), typically 2-4 lanes would be loaded for Western blot. Phosphorylated Aurora A can be difficult to detect, you may want to IP first with pan-Aurora A before blotting with the phospho-specific antibody.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Aurora A (phospho T288) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Aurora A (phospho T288) antibody images, references, products related to ab18318 and other tools.
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Aurora A (phospho T288) antibody images:
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 Western blot - Aurora A (phospho T288) antibody (ab18318)
Predicted band size : 46 kDa
Extracts from untreated Hela cells
(Lane 1) or overnight nocodazole-treated Hela
cells (Lane 2) were immunoprecipitated with a
pan-Aurora A monoclonal antibody (clone 35C1) (available as ab13824), resolved by electrophoresis, transferred
to nitrocellulose and probed with rabbit
polyclonal antibody against Thr288
phosphorylated Aurora A (ab18318). Proteins
were visualized using a donkey anti-rabbit
secondary conjugated to HRP (available as ab16284), and a
chemiluminescence detection system
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- Aurora A (phospho T288) antibody (ab18318)
The blot shown above was stripped and reprobed
with pan-Aurora specific polyclonal
antibody (available as ab12875) to verify equal loading of
Aurora A in the extracts. Proteins were visualized
using a donkey anti-rabbit secondary
conjugated to HRP (available as ab16284)and a chemiluminescence
detection system.
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Immunocytochemistry/ Immunofluorescence - Aurora A (phospho T288) antibody (ab18318)
Prometaphase HeLa cells were permaeabiliased in 0.2% Triton-X100 and fixed for 10 minutes at room temperature in 4% formaldehyde. The cells were washed 3 times in PBS and incubated with ab18318 (1/1000) for 1 hour at room temperature. The panel of images shows the nuceli stained with DAPI (blue) and the centromeres stained with ab18318 (green). 100x magnification. Roberto Giambruno and Giulia Guarguaglini
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References for Aurora A (phospho T288) antibody (ab18318)
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This product has been used in: (two most recent references)
Davids BJ et al. Giardia lamblia aurora kinase: a regulator of mitosis in a binucleate parasite. Int J Parasitol 38:353-69 (2008). ICC/IF. PubMed: 17964578
Uzbekova S et al. Spatio-temporal expression patterns of aurora kinases a, B, and C and cytoplasmic polyadenylation-element-binding protein in bovine oocytes during meiotic maturation. Biol Reprod 78:218-33 (2008). PubMed: 17687118
See all 6 publication references for this product.
If you publish research using ab18318 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Aurora A
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