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Product name
Aurora A protein (His tag)
See all Aurora A products (8) ...
Protein description
Recombinant full length protein (Human)
Expression host
Baculovirus infected sf9 cells
Properties
Purity
> 90% by SDS-PAGE
Biological activity
120 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30?C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 4 uM aurora A substrate, and 0.4 ug/ml Aurora A kinase.
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
Concentration
Concentration information loading...
Additional notes
Expressed in Baculovirus infected Sf9 cell expression system.
Research areas
Cancer >> Tumor biomarkers >> Other
Cancer >> Cell cycle >> Cell division
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Inhibition Assay
Application notes
Inhib: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity (By similarity). Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
Tissue specificity
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome.
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Detected at the neurite hillock in developing neurons (By similarity). Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
Target information above from: UniProt accessionO14965
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for Aurora A protein (His tag) (ab42595)
ab42595 has not yet been referenced specifically in any publications.
Publishing research using ab42595? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"