Anti-Aurora B antibody [EP1009Y] (ab45145)

Overview

• Product nameAnti-Aurora B antibody [EP1009Y]See all Aurora B primary antibodies ...
• Description
  Rabbit monoclonal [EP1009Y] to Aurora B
• Tested applicationsWB, IHC-P, ICC/IF, IP more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide corresponding to residues near the N-terminus of human Aurora B.

• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Dissociation constant (Kd) Kd = 5.50 x 10^-11 M
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1009Y
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Aurora

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Aurora

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Phosphorylation

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Aurora

Applications

Target

• FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
• Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
• Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsUbiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
• Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.

Target information above from: UniProt accession Q96GD4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9212 Human
  • Entrez Gene: 9212 Human
  • Entrez Gene: 9212 Human
  • Omim: 604970 Human
  • SwissProt: Q96GD4 Human
  • SwissProt: Q96GD4 Human
  • SwissProt: Q96GD4 Human
  • Unigene: 442658 Human

• Alternative names
  AIK2 antibodyAIM-1 antibodyAIM1 antibody

  ARK-2 antibodyARK2 antibodyAurB antibodyAURKB antibodyAURKB_HUMAN antibodyAurora 1 antibodyAurora and Ipl1 like midbody associated protein 1 antibodyAurora kinase B antibodyAurora related kinase 2 antibodyAurora- and Ipl1-like midbody-associated protein 1 antibodyAurora-B antibodyAurora-related kinase 2 antibodyAurora/IPL1 related kinase 2 antibodyAurora/IPL1-related kinase 2 antibodyIPL1 antibodySerine/theronine kinase 12 antibodySerine/threonine protein kinase 12 antibodySerine/threonine-protein kinase 12 antibodySerine/threonine-protein kinase aurora-B antibodySTK-1 antibodySTK1 antibodySTK12 antibodySTK5 antibody

  see all

Anti-Aurora B antibody [EP1009Y] images

• 

  Western blot - Aurora B antibody [EP1009Y] (ab45145)
  Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/50000 dilution + HeLa cell lysate
  
  Predicted band size : 39 kDa
  Observed band size : 39 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - Aurora B antibody [EP1009Y] (ab45145)
  Ab45145 staining human Aurora B in human Hodgkin lymphoma by immunohistochemistry using paraffin embedded tissue.
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Aurora B antibody [EP1009Y](ab45145)
  ab45145 showing positive staining in Normal tonsil tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Aurora B antibody [EP1009Y](ab45145)
  ab45145 showing positive staining in Breast carcinoma tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Aurora B antibody [EP1009Y](ab45145)
  ab45145 showing positive staining in Cervical carcinoma tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Aurora B antibody [EP1009Y](ab45145)
  ab45145 showing positive staining in Urinary bladder transitional carcinoma tissue.
  
  
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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [EP1009Y] (ab45145)
  ab45145 showing positive staining in Lung adenocarcinoma tissue.
  
  
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  Other-Anti-Aurora B antibody [EP1009Y](ab45145)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
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