Overview

  • Product nameAnti-Aurora B antibody [EP1009Y]
    See all Aurora B primary antibodies
  • Description
    Rabbit monoclonal [EP1009Y] to Aurora B
  • Tested applicationsWB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human Aurora B.

  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab45145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
IP
  • Application notesICC/IF: 1/100 - 1/150.
    IHC-P: 1/250 - 1/500.
    IP: 1/50.
    WB: 1/50000. Predicted molecular weight: 39 kDa.

    Is unsuitable for FACS.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
    • Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
    • Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
    • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
    • Information by UniProt
    • Database links
    • Alternative names
      • AIK2 antibody
      • AIM-1 antibody
      • AIM1 antibody
      • ARK-2 antibody
      • ARK2 antibody
      • AurB antibody
      • AURKB antibody
      • AURKB_HUMAN antibody
      • Aurora 1 antibody
      • Aurora and Ipl1 like midbody associated protein 1 antibody
      • Aurora kinase B antibody
      • Aurora related kinase 2 antibody
      • Aurora- and Ipl1-like midbody-associated protein 1 antibody
      • Aurora-B antibody
      • Aurora-related kinase 2 antibody
      • Aurora/IPL1 related kinase 2 antibody
      • Aurora/IPL1-related kinase 2 antibody
      • IPL1 antibody
      • Serine/theronine kinase 12 antibody
      • Serine/threonine protein kinase 12 antibody
      • Serine/threonine-protein kinase 12 antibody
      • Serine/threonine-protein kinase aurora-B antibody
      • STK-1 antibody
      • STK1 antibody
      • STK12 antibody
      • STK5 antibody
      see all

    Anti-Aurora B antibody [EP1009Y] images

    • Anti-Aurora B antibody [EP1009Y] (ab45145) at 1/50000 dilution + HeLa cell lysate

      Predicted band size : 39 kDa
      Observed band size : 39 kDa
    • Ab45145 staining human Aurora B in human Hodgkin lymphoma by immunohistochemistry using paraffin embedded tissue.
    • ab45145 showing positive staining in Normal tonsil tissue.

    • ab45145 showing positive staining in Breast carcinoma tissue.

    • ab45145 showing positive staining in Cervical carcinoma tissue.

    • ab45145 showing positive staining in Urinary bladder transitional carcinoma tissue.

    • ab45145 showing positive staining in Lung adenocarcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Aurora B antibody [EP1009Y] (ab45145)

    This product has been referenced in:
    • Chuang MJ  et al. The HDAC inhibitor LBH589 induces ERK-dependent prometaphase arrest in prostate cancer via HDAC6 inactivation and down-regulation. PLoS One 8:e73401 (2013). WB ; Human . Read more (PubMed: 24023871) »
    • Failes TW  et al. Evolution of resistance to Aurora kinase B inhibitors in leukaemia cells. PLoS One 7:e30734 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22359551) »

    See all 7 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"