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RabMAbs (3315-1)

Anti-Aurora B antibody [EPR1010] (ab108331)

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Overview

  • Product nameAnti-Aurora B antibody [EPR1010]See all Aurora B primary antibodies ...
  • Description
    Rabbit monoclonal [EPR1010] to Aurora B
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Aurora B.

  • Positive controlHeLa treated with nocodazole, HeLa, Fetal thymus, and A549 cell lysates
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab108331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
  • Application notesIs unsuitable for ,Flow Cyt,ICC,IHC-P or IP.

    • Target

    • FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
    • Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
    • Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications      Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
    • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.

      • Target information above from: UniProt accession Q96GD4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        AIK2 antibodyAIM-1 antibodyAIM1 antibody
        ARK-2 antibodyARK2 antibodyAurB antibodyAURKB antibodyAURKB_HUMAN antibodyAurora 1 antibodyAurora and Ipl1 like midbody associated protein 1 antibodyAurora kinase B antibodyAurora related kinase 2 antibodyAurora- and Ipl1-like midbody-associated protein 1 antibodyAurora-B antibodyAurora-related kinase 2 antibodyAurora/IPL1 related kinase 2 antibodyAurora/IPL1-related kinase 2 antibodyIPL1 antibodySerine/theronine kinase 12 antibodySerine/threonine protein kinase 12 antibodySerine/threonine-protein kinase 12 antibodySerine/threonine-protein kinase aurora-B antibodySTK-1 antibodySTK1 antibodySTK12 antibodySTK5 antibody
      see all

    Anti-Aurora B antibody [EPR1010] images

    • Western blot - Aurora B antibody [EPR1010] (ab108331)
      Western blot - Aurora B antibody [EPR1010] (ab108331)
      All lanes : Anti-Aurora B antibody [EPR1010] (ab108331) at 1/1000 dilution

      Lane 1 : HeLa treated with nocodazole cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Fetal thymus cell lysate
      Lane 4 : A549 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 39 kDa
      Observed band size : 39 kDa

    References for Anti-Aurora B antibody [EPR1010] (ab108331)

    ab108331 has not yet been referenced specifically in any publications.

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