Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab2254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.

Methanol fixation recommended.

IHC-Fr 1/200.
IHC-P 1/200.
IP Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human Aurora B peptide (ab13569).

Target

  • FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIK2 antibody
    • AIM-1 antibody
    • AIM1 antibody
    • ARK-2 antibody
    • ARK2 antibody
    • AurB antibody
    • AURKB antibody
    • AURKB_HUMAN antibody
    • Aurora 1 antibody
    • Aurora and Ipl1 like midbody associated protein 1 antibody
    • Aurora kinase B antibody
    • Aurora related kinase 2 antibody
    • Aurora- and Ipl1-like midbody-associated protein 1 antibody
    • Aurora-B antibody
    • Aurora-related kinase 2 antibody
    • Aurora/IPL1 related kinase 2 antibody
    • Aurora/IPL1-related kinase 2 antibody
    • IPL1 antibody
    • Serine/theronine kinase 12 antibody
    • Serine/threonine protein kinase 12 antibody
    • Serine/threonine-protein kinase 12 antibody
    • Serine/threonine-protein kinase aurora-B antibody
    • STK-1 antibody
    • STK1 antibody
    • STK12 antibody
    • STK5 antibody
    see all

Anti-Aurora B antibody images

  • ICC/IF image of ab2254 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2254, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • The top panel shows paraformaldehyde fixed HeLa cells stained with ab2254 (1/1000) and counterstained with DAPI (red). Staining with ab2254 is shown in green. In the lower panel the staining with ab2254 is quenched by the addition of the blocking peptide, ab13569.

  • The top panel shows paraformaldehyde fixed HeLa cells stained with ab2254 (1/1000) and counterstained with DAPI (red). Staining with ab2254 is shown in green. In the lower panel the staining with ab2254 is quenched by the addition of the blocking peptide, ab13569.

  • ab2254 staining human A431 (epithelial) cells by ICC/IF.  The sample was fixed in paraformaldehyde and permeabilized by incubation with 0.1% Triton X100.  1% BSA was used as the blocking agent prior to a 1 hour incubation with the primary antibody, diluted 1/1000 with 1% BSA made up in PBS.  An Alexa Fluor® 647 conjugated Donkey anti-Rabbit IgG (H+L) antibody was used as the secondary.  Blocking and antibody incubation steps were carried out at room temperature.

    In this set of images, the tubulin is stained green, Aurora B in pink and DNA in blue.

    See Abreview

  • Immunofluorescence in human cells using Rabbit polyclonal to Aurora B (red), DAPI (blue) and CREST serum (binds to centromeres)(green).

    (a) HeLa cells - transition from interphase (left) through mitosis
    (b) RPE-1 cells - as in (a)
    (c) HeLa cells - interphase
    (d) RPE-1 cells - interphase

  • Rabbit polyclonal to Aurora B (ab2254) used to stain SW620 human tumour xenografts (in mouse).

    The sections were microwave pretreated in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave).  Slides were then incubated for 1 hour with the Aurora B primary antibody diluted 1/200 in TBS, then visualised using DAB, after application of an appropriate secondary. 


  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
  • All lanes : Anti-Aurora B antibody (ab2254) at 1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate
    Lane 2 : HeLa Nuclear Lysate
    Lane 3 : Jurkat Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 37 kDa (possible isoform).

    Exposure time : 150 seconds

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa

References for Anti-Aurora B antibody (ab2254)

This product has been referenced in:
  • Balboula AZ & Schindler K Selective disruption of aurora C kinase reveals distinct functions from aurora B kinase during meiosis in mouse oocytes. PLoS Genet 10:e1004194 (2014). ICC/IF ; Mouse . Read more (PubMed: 24586209) »
  • Banerjee B  et al. EB1 enables spindle microtubules to regulate centromeric recruitment of Aurora B. J Cell Biol 204:947-63 (2014). Human . Read more (PubMed: 24616220) »

See all 50 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.3% Triton X
Fixative Methanol
Username

Abcam user community

Verified customer

Submitted Oct 08 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Sample Human Tissue sections (colorectal carcinoma)
Specification colorectal carcinoma
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 08 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 08 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - nuclear (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Apr 18 2014

Thank you for your co-operation in this matter.

There may well be batch-to-batch variations since this is a polyclonal antibody and the immune response of rabbits used for immunization can be different. We would advise our customers to optimi...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human colon carcinoma)
Loading amount 50 µg
Specification Human colon carcinoma
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 25 2012

Thank you for your enquiry and your interest in our products.

I have run a blast search and it seems only the sickie, isoform B in Drosophila melanogaster shares some sequence homology but no data available for the whole protein. There seems ...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 75 µg
Gel Running Conditions Non-reduced Denaturing (10 %)
Sample Human Cell lysate - whole cell (Colon carcinoma)
Specification Colon carcinoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

Submitted Oct 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"