Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Aurora B antibody
See all Aurora B products (15) ...
Description
Rabbit polyclonal to Aurora B
Tested applications
ICC/IF, IHC-Fr, IF, IHC-P, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Hamster, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Aurora B.
(Peptide available as ab135 69.)
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Aurora
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Phosphorylation
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Aurora
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: 1/200.
IF
IF: 1/1000 - 1/2000.IF: 1/1000 - 1/2000.
IHC-P: 1/200.
IP: Use at an assay dependent dilution.
WB: 1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Aurora B peptide (ab13569).
Target
Function
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
Tissue specificity
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Involvement in disease
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
Target information above from: UniProt accessionQ96GD4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
Database links
- Entrez Gene: 9212 Human
- Entrez Gene: 20877 Mouse
- Entrez Gene: 114592 Rat
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- SwissProt: O70126 Mouse
- SwissProt: O55099 Rat
- Unigene: 442658 Human
see all
Anti-Aurora B antibody images:
Immunocytochemistry/ Immunofluorescence
The top panel shows paraformaldehyde fixed HeLa cells stained with ab2254 (1/1000) and counterstained with DAPI (red). Staining with ab2254 is shown in green. In the lower panel the staining with ab2254 is quenched by the addition of the blocking peptide, ab13569.
Kirk McManus, University of British Columbia
Immunocytochemistry/ Immunofluorescence - Aurora B antibody (ab2254)
ab2254 staining human A431 (epithelial) cells by ICC/IF. The sample was fixed in paraformaldehyde and permeabilized by incubation with 0.1% Triton X100. 1% BSA was used as the blocking agent prior to a 1 hour incubation with the primary antibody, diluted 1/1000 with 1% BSA made up in PBS. An Alexa Fluor® 647 conjugated Donkey anti-Rabbit IgG (H+L) antibody was used as the secondary. Blocking and antibody incubation steps were carried out at room temperature.
In this set of images, the tubulin is stained green, Aurora B in pink and DNA in blue.
This image is courtesy of an Abreview from Lux Fatimathas.
Immunocytochemistry/ Immunofluorescence - Aurora B antibody (ab2254)
Immunofluorescence in human cells using Rabbit polyclonal to Aurora B (red), DAPI (blue) and CREST serum (binds to centromeres)(green).
(a) HeLa cells - transition from interphase (left) through mitosis
(b) RPE-1 cells - as in (a)
(c) HeLa cells - interphase
(d) RPE-1 cells - interphase
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aurora B antibody (ab2254)
Rabbit polyclonal to Aurora B (ab2254) used to stain SW620 human tumour xenografts (in mouse).
The sections were microwave pretreated in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). Slides were then incubated for 1 hour with the Aurora B primary antibody diluted 1/200 in TBS, then visualised using DAB, after application of an appropriate secondary.
Western blot - Aurora B antibody [mAbcam 10735] (ab10735)
![Western blot - Aurora B antibody [mAbcam 10735] (ab10735)](/ps/datasheet/Images/10/ab10735/ab10735_2.jpg)
Lanes 1 - 2 : Anti-Aurora B antibody [mAbcam 10735] (ab10735) at 1 µg/ml
Lanes 3 - 4 : Anti-Aurora B antibody (ab2254) at 1 µg/ml
Lane 1 : Hela whole cell lysate
Lane 2 : Hela whole cell lysate with
Lane 3 : Hela whole cell lysate
Lane 4 : Hela whole cell lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789) at 1/5000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 39 kDa
Exposure time : 2 minutes
Immunocytochemistry/ Immunofluorescence - Aurora B antibody (ab2254)
ICC/IF image of ab2254 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2254, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Aurora B antibody (ab2254)
Anti-Aurora B antibody (ab2254) at 1/2000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 30 seconds
References for Anti-Aurora B antibody (ab2254)
This product has been referenced in:
- von Gise A et al. YAP1, the nuclear target of Hippo signaling, stimulates heart growth through cardiomyocyte proliferation but not hypertrophy. Proc Natl Acad Sci U S A 109:2394-9 (2012). Mouse.Read more (PubMed: 22308401) »
- Tardáguila M et al. Aurora kinase B activity is modulated by thyroid hormone during transcriptional activation of pituitary genes. Mol Endocrinol 25:385-93 (2011). WB.Read more (PubMed: 21239609) »
See all 38 publications for this product
Publishing research using ab2254? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"