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Overview

  • Product nameAnti-Aurora B antibodySee all Aurora B primary antibodies ...
  • Description
    Rabbit polyclonal to Aurora B
  • SpecificityDetects endogenous levels of total Aurora B protein
  • Tested applicationsWB, ICC/IF, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide (Human) from around the phosphorylation site of threonine 232 (RKTPMC)

  • Positive controlHeLa cells Cos 7 cells treated with Nocodazole

Properties

Applications

Our Abpromise guarantee covers the use of ab61188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF ICC/IF: 1/500 - 1/1000.
ELISA ELISA: 1/5000.

Target

  • FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.

    • Target information above from: UniProt accession Q96GD4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      AIK2 antibodyAIM-1 antibodyAIM1 antibody
      ARK-2 antibodyARK2 antibodyAurB antibodyAURKB antibodyAURKB_HUMAN antibodyAurora 1 antibodyAurora and Ipl1 like midbody associated protein 1 antibodyAurora kinase B antibodyAurora related kinase 2 antibodyAurora- and Ipl1-like midbody-associated protein 1 antibodyAurora-B antibodyAurora-related kinase 2 antibodyAurora/IPL1 related kinase 2 antibodyAurora/IPL1-related kinase 2 antibodyIPL1 antibodySerine/theronine kinase 12 antibodySerine/threonine protein kinase 12 antibodySerine/threonine-protein kinase 12 antibodySerine/threonine-protein kinase aurora-B antibodySTK-1 antibodySTK1 antibodySTK12 antibodySTK5 antibody
    see all

Anti-Aurora B antibody images

  • Western blot - Aurora B antibody (ab61188)
    Western blot - Aurora B antibody (ab61188)
    All lanes : Anti-Aurora B antibody (ab61188) at 1/500 dilution

    Lane 1 : Cos7 cell extracts treated with Nocodazole (1 microgram/ml, 16 hours)
    Lane 2 : Cos7 cell extracts treated with Nocodazole (1 microgram/ml, 16 hours) with peptide


    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Immunocytochemistry/ Immunofluorescence - Aurora B antibody (ab61188)
    Immunocytochemistry/ Immunofluorescence - Aurora B antibody (ab61188)
    Immunofluorescence analysis of HeLa cells labelled with ab61188 at 1/500 dilution. Samples were treated -/+ peptide.

References for Anti-Aurora B antibody (ab61188)

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10
Votes: Highest First

Hi,
Could you share me the % identities and number of gaps of the BLASTp between the precise recognized epitope by your Anti-AURKB, ab2254
as well as that of the ab61188, ab62702, ab70238, ab45145, and ab3609 against the sequence of the bovine A...

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This immunogen sequence has 100% identity with the bovine AURKB (uniprot Q7YRC6).

I hope this is helpful. Please contact me again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"