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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Aurora
Overview
Product name
Anti-Aurora B antibody
See all Aurora B products (15) ...
Description
Rabbit polyclonal to Aurora B
Specificity
ab62702 detects endogenous levels of total Aurora B protein.
Tested applications
ELISA, IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non phosphopeptide derived from human Aurora B around the phosphorylation site of tyrosine 12 (WPYPGR).
Positive control
Raw264.7 cell extracts treated with Hu (2nM, 24hours), HeLa cells treated with Forskolin (40nM, 30mins) and human liver carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab62702 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Aurora
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Phosphorylation
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Aurora
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ICC/IF: 1/500 - 1/1000.
Target
Function
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
Tissue specificity
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Involvement in disease
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
Target information above from: UniProt accessionQ96GD4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
Database links
- Entrez Gene: 9212 Human
- Entrez Gene: 20877 Mouse
- Entrez Gene: 114592 Rat
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- SwissProt: O70126 Mouse
- SwissProt: O55099 Rat
- Unigene: 442658 Human
see all
Anti-Aurora B antibody images:
Western blot - Aurora B antibody (ab62702)
All lanes : Anti-Aurora B antibody (ab62702) at 1/500 dilution
Lane 1 : Raw264.7 cell extracts treated with Hu (2nM, 24hours)
Lane 2 : Raw264.7 cell extracts treated with Hu (2nM, 24hours) with immunising peptide
Predicted band size : 39 kDa
Observed band size : 39 kDa
Immunocytochemistry/ Immunofluorescence - Aurora B antibody (ab62702)
ab62702 at 1/500 - 1/1000 dilution staining Aurora B in HeLa cells, treated with Forskolin (40nM, 30mins) by Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aurora B antibody (ab62702)
ab62702 at 1/50 - 1/100 dilution staining Aurora B in human liver carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
References for Anti-Aurora B antibody (ab62702)
ab62702 has not yet been referenced specifically in any publications.
Publishing research using ab62702? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"