Overview

  • Product nameAnti-Aurora B antibody
    See all Aurora B primary antibodies
  • Description
    Rabbit polyclonal to Aurora B
  • Tested applicationsWB, IP, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 300 and the C-terminus (residue 344) of Human Aurora B (Swiss-Prot Q96GD4).

Properties

Applications

Our Abpromise guarantee covers the use of ab70238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IP Use at 1-4 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration. PubMed: 21844210
ICC 1/500 - 1/2000.

Target

  • FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIK2 antibody
    • AIM-1 antibody
    • AIM1 antibody
    • ARK-2 antibody
    • ARK2 antibody
    • AurB antibody
    • AURKB antibody
    • AURKB_HUMAN antibody
    • Aurora 1 antibody
    • Aurora and Ipl1 like midbody associated protein 1 antibody
    • Aurora kinase B antibody
    • Aurora related kinase 2 antibody
    • Aurora- and Ipl1-like midbody-associated protein 1 antibody
    • Aurora-B antibody
    • Aurora-related kinase 2 antibody
    • Aurora/IPL1 related kinase 2 antibody
    • Aurora/IPL1-related kinase 2 antibody
    • IPL1 antibody
    • Serine/theronine kinase 12 antibody
    • Serine/threonine protein kinase 12 antibody
    • Serine/threonine-protein kinase 12 antibody
    • Serine/threonine-protein kinase aurora-B antibody
    • STK-1 antibody
    • STK1 antibody
    • STK12 antibody
    • STK5 antibody
    see all

Anti-Aurora B antibody images

  • Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.
  • All lanes : Anti-Aurora B antibody (ab70238) at 1/10000 dilution

    Lane 1 : Whole cell lysate from non-adherent HeLa cells treated with nocodazole (20h)
    Lane 2 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)
    Lane 3 : Whole cell lysate from non-adherent HeLa cells treated with nocodazole (20h)
    Lane 4 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)

    Lysates/proteins at 50 µg per lane.

    developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 28 kDa,36 kDa. We are unsure as to the identity of these extra bands.
    Detection time: 10 secs (lanes 1,2) or 30 secs (lanes 3,4)
  • All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate from an asynchronous culture
    Lane 2 : HeLa whole cell lysate treated with cycloheximide for 12 hours
    Lane 3 : HeLa whole cell lysatetreated with cycloheximide for 24 hours
    Lane 4 : HeLa whole cell lysate treated with cycloheximide for 36 hours
    Lane 5 : HeLa whole cell lysate treated with nocodazole for 20 hours

    Lysates/proteins at 100 µg per lane.


    Predicted band size : 39 kDa

References for Anti-Aurora B antibody (ab70238)

This product has been referenced in:
  • Martz MK  et al. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Mol Biol Cell 24:2785-94 (2013). Read more (PubMed: 23885121) »
  • Wang E  et al. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol 194:539-49 (2011). ICC/IF ; Human . Read more (PubMed: 21844210) »

See all 2 Publications for this product

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