![Western blot - Aurora B antibody [mAbcam 10735] (ab10735) image](#ab10735_2.jpg)
 image](#Aurora-B-Primary-antibodies-ab10735-1.jpg)
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Product name
Anti-Aurora B antibody [mAbcam 10735]
See all Aurora B products (15) ...
Description
Mouse monoclonal [mAbcam 10735] to Aurora B
Tested applications
Flow Cyt, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Aurora B.
(Peptide available as ab135 69.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, Tris HCl, 500mM Sodium chloride, 200mM Sodium citrate. pH 7.5
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
mAbcam 10735
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Aurora
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Phosphorylation
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Aurora
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 2µg for 106 cells.
WB: Use at an assay dependent dilution. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Target
Function
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
Tissue specificity
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Involvement in disease
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
Target information above from: UniProt accessionQ96GD4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
Database links
- Entrez Gene: 9212 Human
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- Unigene: 442658 Human
Anti-Aurora B antibody [mAbcam 10735] images:
Western blot - Aurora B antibody [mAbcam 10735] (ab10735)
![Western blot - Aurora B antibody [mAbcam 10735] (ab10735)](/ps/datasheet/Images/10/ab10735/ab10735_2.jpg)
Lanes 1 - 2 : Anti-Aurora B antibody [mAbcam 10735] (ab10735) at 1 µg/ml
Lanes 3 - 4 : Anti-Aurora B antibody (ab2254) at 1 µg/ml
Lane 1 : Hela whole cell lysate
Lane 2 : Hela whole cell lysate with
Lane 3 : Hela whole cell lysate
Lane 4 : Hela whole cell lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789) at 1/5000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 39 kDa
Exposure time : 2 minutes
Flow Cytometry-Anti-Aurora B antibody [mAbcam 10735](ab10735)
](/ps/datasheet/images/10/ab10735/Aurora-B-Primary-antibodies-ab10735-1.jpg)
Overlay histogram showing HeLa cells stained with ab10735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10735, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-Aurora B antibody [mAbcam 10735] (ab10735)
ab10735 has not yet been referenced specifically in any publications.
Publishing research using ab10735? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"