Anti-Aurora B antibody [mAbcam 10735] (ab10735)

Overview

• Product nameAnti-Aurora B antibody [mAbcam 10735]See all Aurora B primary antibodies ...
• Description
  Mouse monoclonal [mAbcam 10735] to Aurora B
• Tested applicationsFlow Cyt, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Aurora B.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab13569.)

• Positive controlIn Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HeLa cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, Tris HCl, 500mM Sodium chloride, 200mM Sodium citrate. pH 7.5
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numbermAbcam 10735
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Aurora

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Aurora

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Phosphorylation

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Aurora

Applications

Target

• FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
• Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
• Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsUbiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
• Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.

Target information above from: UniProt accession Q96GD4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9212 Human
  • Entrez Gene: 9212 Human
  • Entrez Gene: 9212 Human
  • Omim: 604970 Human
  • SwissProt: Q96GD4 Human
  • SwissProt: Q96GD4 Human
  • SwissProt: Q96GD4 Human
  • Unigene: 442658 Human

• Alternative names
  AIK2 antibodyAIM-1 antibodyAIM1 antibody

  ARK-2 antibodyARK2 antibodyAurB antibodyAURKB antibodyAURKB_HUMAN antibodyAurora 1 antibodyAurora and Ipl1 like midbody associated protein 1 antibodyAurora kinase B antibodyAurora related kinase 2 antibodyAurora- and Ipl1-like midbody-associated protein 1 antibodyAurora-B antibodyAurora-related kinase 2 antibodyAurora/IPL1 related kinase 2 antibodyAurora/IPL1-related kinase 2 antibodyIPL1 antibodySerine/theronine kinase 12 antibodySerine/threonine protein kinase 12 antibodySerine/threonine-protein kinase 12 antibodySerine/threonine-protein kinase aurora-B antibodySTK-1 antibodySTK1 antibodySTK12 antibodySTK5 antibody

  see all

Anti-Aurora B antibody [mAbcam 10735] images

• 

  Western blot - Aurora B antibody [mAbcam 10735] (ab10735)
  Lanes 1 - 2 : Anti-Aurora B antibody [mAbcam 10735] (ab10735) at 1 µg/ml
  Lanes 3 - 4 : Anti-Aurora B antibody (ab2254) at 1 µg/ml
  
  Lane 1 : Hela whole cell lysate
  Lane 2 : Hela whole cell lysate with Aurora B peptide (ab13569) at 1 µg/ml
  Lane 3 : Hela whole cell lysate
  Lane 4 : Hela whole cell lysate with Aurora B peptide (ab13569) at 1 µg/ml
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789) at 1/5000 dilution
  Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
  
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Observed band size : 39 kDa
  
  
  Exposure time : 2 minutes
• 

  Flow Cytometry-Anti-Aurora B antibody [mAbcam 10735](ab10735)
  Overlay histogram showing HeLa cells stained with ab10735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10735, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.