You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-Aurora B antibody [mAbcam 3609] (ab3609)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-Aurora B antibody [mAbcam 3609]
See all Aurora B products (15) ...

Description

Mouse monoclonal [mAbcam 3609] to Aurora B

Tested applications

WB, ICC/IF, IHC-Pmore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Aurora B.

Read Abcam's proprietary immunogen policy

(Peptide available as ab135 69.)

Positive control

Hela whole cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

mAbcam 3609

Isotype

IgG1

Light chain type

kappa

Research areas

Cancer >> Cell cycle >> Kinases/phosphatases >> Aurora
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Phosphorylation
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Aurora

  • Western blot - Aurora B antibody [mAbcam 3609] (ab3609)Western blot - Aurora B antibody [mAbcam 3609] (ab3609) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Aurora B antibody [mAbcam 3609] (ab3609)Immunocytochemistry/ Immunofluorescence - Aurora B antibody [mAbcam 3609] (ab3609) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - Aurora B antibody [mAbcam 3609] (ab3609)Immunohistochemistry (Paraffin-embedded sections) - Aurora B antibody [mAbcam 3609] (ab3609) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.

Tissue specificity

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.

Involvement in disease

Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.

Post-translational
modifications

Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.

Cellular localization

Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.

Target information above from: UniProt accessionQ96GD4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AIK2 antibody
  • AIM-1 antibody
  • AIM1 antibody
  • ARK-2 antibody
  • ARK2 antibody
  • AurB antibody
  • AURKB antibody
  • AURKB_HUMAN antibody
  • Aurora 1 antibody
  • Aurora and Ipl1 like midbody associated protein 1 antibody
  • Aurora kinase B antibody
  • Aurora related kinase 2 antibody
  • Aurora- and Ipl1-like midbody-associated protein 1 antibody
  • Aurora-B antibody
  • Aurora-related kinase 2 antibody
  • Aurora/IPL1 related kinase 2 antibody
  • Aurora/IPL1-related kinase 2 antibody
  • IPL1 antibody
  • Serine/theronine kinase 12 antibody
  • Serine/threonine protein kinase 12 antibody
  • Serine/threonine-protein kinase 12 antibody
  • Serine/threonine-protein kinase aurora-B antibody
  • STK-1 antibody
  • STK1 antibody
  • STK12 antibody
  • STK5 antibody
see all

Database links

see all

Anti-Aurora B antibody [mAbcam 3609] images:

  Western blot - Aurora B antibody [mAbcam 3609] (ab3609)

Western blot - Aurora B antibody [mAbcam 3609] (ab3609)

Anti-Aurora B antibody [mAbcam 3609] (ab3609) at 1/1000 dilution + HeLa whole cell lysate at 1 µg/ml

Performed under reducing conditions.

Predicted band size : 39 kDa


Blocked with 1%BSA/TBST.

  Immunocytochemistry/ Immunofluorescence - Aurora B antibody [mAbcam 3609] (ab3609)

Immunocytochemistry/ Immunofluorescence - Aurora B antibody [mAbcam 3609] (ab3609)

SKN-SH cells were fixed in 4% paraformaldehyde for 10 mins, permeabilized in PBS-0.5% Triton X-100 for 5 mins and incubated for 30 minutes with ab3609 (1/50 dilution). The slides were blocked with 4mg/ml BSA. The slides were rinsed once in PBS-Triton (0.1%), twice in PBS then incubated with the secondary antibody for 30 mins. The DNA is stained with DAPI (blue). Staining with ab3609 can be seen in green.

  Immunohistochemistry (Paraffin-embedded sections) - Aurora B antibody [mAbcam 3609] (ab3609)

Immunohistochemistry (Paraffin-embedded sections) - Aurora B antibody [mAbcam 3609] (ab3609)

Image courtesy of Human Protein Atlas

Paraffin embedded human liver tumor tissue was incubated with ab3609 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

ab3609 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org

  Immunocytochemistry/ Immunofluorescence - Aurora B antibody [mAbcam 3609] (ab3609)

Immunocytochemistry/ Immunofluorescence - Aurora B antibody [mAbcam 3609] (ab3609)

ab3609 (1/200) staining Aurora B in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus/ chromosomes (red). For further experimental details please refer to Abreview.

Image courtesy of an abreview submitted by Dr. Kirk McManus, University of Manitoba/MICB, Canada

See Abreview

References for Anti-Aurora B antibody [mAbcam 3609] (ab3609)

ab3609 has not yet been referenced specifically in any publications.

Publishing research using ab3609? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"