Anti-Aurora B antibody [mAbcam 3609] (ab3609)

Overview

• Product nameAnti-Aurora B antibody [mAbcam 3609]See all Aurora B primary antibodies ...
• Description
  Mouse monoclonal [mAbcam 3609] to Aurora B
• Tested applicationsWB, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Rat, Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Aurora B.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab13569.)

• Positive controlHela whole cell lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numbermAbcam 3609
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Aurora

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Aurora

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Phosphorylation

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Aurora

Applications

Target

• FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
• Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
• Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsUbiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
• Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.

Target information above from: UniProt accession Q96GD4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9212 Human
  • Entrez Gene: 9212 Human
  • Entrez Gene: 9212 Human
  • Entrez Gene: 20877 Mouse
  • Entrez Gene: 114592 Rat
  • Omim: 604970 Human
  • SwissProt: Q96GD4 Human
  • SwissProt: Q96GD4 Human

  • SwissProt: Q96GD4 Human
  • SwissProt: O70126 Mouse
  • SwissProt: O55099 Rat
  • Unigene: 442658 Human
  • Unigene: 3488 Mouse
  • Unigene: 10865 Rat

  see all

• Alternative names
  AIK2 antibodyAIM-1 antibodyAIM1 antibody

  ARK-2 antibodyARK2 antibodyAurB antibodyAURKB antibodyAURKB_HUMAN antibodyAurora 1 antibodyAurora and Ipl1 like midbody associated protein 1 antibodyAurora kinase B antibodyAurora related kinase 2 antibodyAurora- and Ipl1-like midbody-associated protein 1 antibodyAurora-B antibodyAurora-related kinase 2 antibodyAurora/IPL1 related kinase 2 antibodyAurora/IPL1-related kinase 2 antibodyIPL1 antibodySerine/theronine kinase 12 antibodySerine/threonine protein kinase 12 antibodySerine/threonine-protein kinase 12 antibodySerine/threonine-protein kinase aurora-B antibodySTK-1 antibodySTK1 antibodySTK12 antibodySTK5 antibody

  see all

Anti-Aurora B antibody [mAbcam 3609] images

• 

  Western blot - Aurora B antibody [mAbcam 3609] (ab3609)
  Anti-Aurora B antibody [mAbcam 3609] (ab3609) at 1/1000 dilution + HeLa whole cell lysate at 1 µg/ml
  
  Performed under reducing conditions.
  
  Predicted band size : 39 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Aurora B antibody [mAbcam 3609] (ab3609)

  SKN-SH cells were fixed in 4% paraformaldehyde for 10 mins, permeabilized in PBS-0.5% Triton X-100 for 5 mins and incubated for 30 minutes with ab3609 (1/50 dilution). The slides were blocked with 4mg/ml BSA. The slides were rinsed once in PBS-Triton (0.1%), twice in PBS then incubated with the secondary antibody for 30 mins. The DNA is stained with DAPI (blue). Staining with ab3609 can be seen in green.

• 

  Immunohistochemistry (Paraffin-embedded sections) - Aurora B antibody [mAbcam 3609] (ab3609)
  Image courtesy of Human Protein Atlas

  Paraffin embedded human liver tumor tissue was incubated with ab3609 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

  ab3609 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org

• 

  Immunocytochemistry/ Immunofluorescence - Aurora B antibody [mAbcam 3609] (ab3609)Image courtesy of an abreview submitted by Dr. Kirk McManus, University of Manitoba/MICB, Canada
  ab3609 (1/200) staining Aurora B in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus/ chromosomes (red). For further experimental details please refer to Abreview.

  See Abreview