Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Aurora
Overview
Product name
Anti-Aurora B (phospho T232) antibody
See all Aurora B products (15) ...
Description
Rabbit polyclonal to Aurora B (phospho T232)
Specificity
ab61074 detects endogenous levels of AurB only when phosphorylated at threonine 232.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic phosphopeptide derived from human Aurora B around the phosphorylation site of threonine 232 (R-K-TP-M-C).
Positive control
Extracts from COS7 cells, starved in serum-free media for 18-24 hours then treated with Nocodazole (1ug/ml in fresg serum-free media) for 16 hours.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Aurora
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Aurora
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Phosphorylation
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Aurora
-
Western blot - Aurora B (phospho T232) antibody (ab61074)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Function
May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
Tissue specificity
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Involvement in disease
Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
Cellular localization
Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
Target information above from: UniProt accessionQ96GD4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIK2 antibody
- AIM-1 antibody
- AIM1 antibody
see all
Database links
- Entrez Gene: 9212 Human
- Entrez Gene: 20877 Mouse
- Entrez Gene: 114592 Rat
- Omim: 604970 Human
- SwissProt: Q96GD4 Human
- SwissProt: O70126 Mouse
- SwissProt: O55099 Rat
- Unigene: 442658 Human
see all
Anti-Aurora B (phospho T232) antibody images:
Western blot - Aurora B (phospho T232) antibody (ab61074)
All lanes : Anti-Aurora B (phospho T232) antibody (ab61074) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated with Nocodazole (1ug/ml, 16hours)
Lane 2 : Extracts from COS7 cells, treated with Nocodazole (1ug/ml, 16hours) and with the immunising phospho-peptide.
Predicted band size : 39 kDa
Observed band size : 39 kDa
References for Anti-Aurora B (phospho T232) antibody (ab61074)
This product has been referenced in:
- Fichter CDet al. Occurrence of multipolar mitoses and association with Aurora-A/-B kinases and p53 mutations in aneuploid esophageal carcinoma cells. BMC Cell Biol 12:13 (2011). WB; Human.Read more (PubMed: 21470402) »
See 1 publication for this product
Publishing research using ab61074? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"