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Overview

  • Product nameAnti-Aurora B (phospho T232) antibodySee all Aurora B primary antibodies ...
  • Description
    Rabbit polyclonal to Aurora B (phospho T232)
  • Specificityab61074 detects endogenous levels of AurB only when phosphorylated at threonine 232.
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Aurora B around the phosphorylation site of threonine 232 (R-K-TP-M-C).

  • Positive controlExtracts from COS7 cells, starved in serum-free media for 18-24 hours then treated with Nocodazole (1ug/ml in fresg serum-free media) for 16 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab61074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA ELISA: 1/20000.

Target

  • FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.

    • Target information above from: UniProt accession Q96GD4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      AIK2 antibodyAIM-1 antibodyAIM1 antibody
      ARK-2 antibodyARK2 antibodyAurB antibodyAURKB antibodyAURKB_HUMAN antibodyAurora 1 antibodyAurora and Ipl1 like midbody associated protein 1 antibodyAurora kinase B antibodyAurora related kinase 2 antibodyAurora- and Ipl1-like midbody-associated protein 1 antibodyAurora-B antibodyAurora-related kinase 2 antibodyAurora/IPL1 related kinase 2 antibodyAurora/IPL1-related kinase 2 antibodyIPL1 antibodySerine/theronine kinase 12 antibodySerine/threonine protein kinase 12 antibodySerine/threonine-protein kinase 12 antibodySerine/threonine-protein kinase aurora-B antibodySTK-1 antibodySTK1 antibodySTK12 antibodySTK5 antibody
    see all

Anti-Aurora B (phospho T232) antibody images

  • Western blot - Aurora B (phospho T232) antibody (ab61074)
    Western blot - Aurora B (phospho T232) antibody (ab61074)
    All lanes : Anti-Aurora B (phospho T232) antibody (ab61074) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells treated with Nocodazole (1ug/ml, 16hours)
    Lane 2 : Extracts from COS7 cells, treated with Nocodazole (1ug/ml, 16hours) and with the immunising phospho-peptide.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa

References for Anti-Aurora B (phospho T232) antibody (ab61074)

This product has been referenced in:
  • Fichter CD  et al. Occurrence of multipolar mitoses and association with Aurora-A/-B kinases and p53 mutations in aneuploid esophageal carcinoma cells. BMC Cell Biol 12:13 (2011). WB ; Human . Read more (PubMed: 21470402) »

See 1 Publication for this product

Publishing research using ab61074 ? Please let us know so that we can cite the reference in this datasheet

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Having difficulties with background wtih ab61074
Mosue samples
What protocol was used to test in WB?

Thank you once again for your recent telephone enquiry.

As requested, I am forwarding a copy of the western blot protocol which I hope will be helpful to you.

Please note, this would be a guideline only and individual optimization ma...

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Having difficulties with background wtih ab61074
Mosue samples
What protocol was used to test in WB?

Thank you for taking the time to telephone us with your enquiry yesterday afternoon. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and co...

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Testing discount code to try antibody in IF.

DISCOUNT CODE: *******
Expiration date: *************

I am very pleased to hear you would like to accept our offer and test ab61074 in IF in human tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redee...

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Our customer explained his data showed Nocodazole treatedHela cells and P-H3 used as a positive control.
Because he set P-H3 as a positive control and checked bands on 8˜9 hr, he thinks p-Aurora B should detected.
Please let me know if you need ...

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Thank you for liaising with the customer and updating us. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed the protocol details with the confi...

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DESCRIPTION OF THE PROBLEM No signal or weak signal
SAMPLE HeLa cell (human)
PRIMARY ANTIBODY Concentration or dilution 1:1000 Diluent buffer 3% BSA TBST buffer Incubation time over night Incubation temperature: 4℃ Buffer TBST Number of washes 3...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

Having reviewed this case, I would like to offerthe following suggestion: I would also appreciate if you can c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"