Anti-Aurora B (phospho Y12) antibody (ab62466)

Overview

• Product nameAnti-Aurora B (phospho Y12) antibodySee all Aurora B primary antibodies ...
• Description
  Rabbit polyclonal to Aurora B (phospho Y12)
• Specificityab62466 detects endogenous levels of Aurora B only when phosphorylated at tyrosine 12.
• Tested applicationsELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic phosphopeptide derived from human Aurora B around the phosphorylation site of tyrosine 12 (W-P-Y^P-G-R).

• Positive controlHuman liver carcinoma.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab62466 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Aurora

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Aurora

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Phosphorylation

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Aurora

Applications

Target

• FunctionMay be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
• Tissue specificityHigh level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
• Involvement in diseaseNote=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsUbiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
• Cellular localizationNucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.

Target information above from: UniProt accession Q96GD4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9212 Human
  • Entrez Gene: 9212 Human
  • Entrez Gene: 9212 Human
  • Entrez Gene: 20877 Mouse
  • Entrez Gene: 114592 Rat
  • Omim: 604970 Human
  • SwissProt: Q96GD4 Human
  • SwissProt: Q96GD4 Human

  • SwissProt: Q96GD4 Human
  • SwissProt: O70126 Mouse
  • SwissProt: O55099 Rat
  • Unigene: 442658 Human
  • Unigene: 3488 Mouse
  • Unigene: 10865 Rat

  see all

• Alternative names
  AIK2 antibodyAIM-1 antibodyAIM1 antibody

  ARK-2 antibodyARK2 antibodyAurB antibodyAURKB antibodyAURKB_HUMAN antibodyAurora 1 antibodyAurora and Ipl1 like midbody associated protein 1 antibodyAurora kinase B antibodyAurora related kinase 2 antibodyAurora- and Ipl1-like midbody-associated protein 1 antibodyAurora-B antibodyAurora-related kinase 2 antibodyAurora/IPL1 related kinase 2 antibodyAurora/IPL1-related kinase 2 antibodyIPL1 antibodySerine/theronine kinase 12 antibodySerine/threonine protein kinase 12 antibodySerine/threonine-protein kinase 12 antibodySerine/threonine-protein kinase aurora-B antibodySTK-1 antibodySTK1 antibodySTK12 antibodySTK5 antibody

  see all

Anti-Aurora B (phospho Y12) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Aurora B (phospho Y12) antibody (ab62466)
  ab62466, at 1/50 dilution, staining Aurora B in human liver carcinoma tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.